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CASP3F

Sigma-Aldrich

Caspase 3 Assay Kit, Fluorimetric

Synonyme(s) :

Caspase-3 Activity Fluorometric Kit

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About This Item

Numéro CE :
247-454-8
Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 1,000 multiwell tests
 sufficient for 100 standard tests (2 ml)

Niveau de qualité

200

Conditions d'expédition

dry ice

Température de stockage

−20°C

Informations sur le gène

human ... CASP3(836)

Description générale

The Caspase 3 Fluorometric Assay Kit is based on the hydrolysis of acetyl Asp-Glu-Val-Asp 7-amido-4-methylcoumarin (Ac-DEVD-AMC) by caspase 3, resulting in the release of the fluorescent 7-amino-4-methylcoumarin (AMC). The excitation and emission wavelengths of AMC are 360 nm and 460 nm respectively. The concentration of the AMC released can be calculated from a calibration curve prepared with AMC standards (AMC standard included with the kit).

Application

The Caspase 3 Fluorimetric Assay Kit is designed for the detection and measurement of caspase 3 activity in cell extracts or purified preparations of caspase 3.

Définition de l'unité

One unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD per min at pH 7.4 at 25 °C.

Composants de kit seuls

Réf. du produit
Description
  • Water (17-18 megaOhm) 125 mL

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS
  • A1086Ac-DEVD-AMC substrate 2.5 mgFDS
  • A0835Ac-DEVD-CHO inhibitor .5 mgFDS
  • A98917-Amino-4-methylcoumarin standard 1 mgFDS

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H319

Classification des risques

Eye Irrit. 2

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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