Macrophage receptor MARCO (UniProt: Q9UEW3; also known as macrophage receptor with collagenous structure; Scavenger receptor class A member 2) is encoded by the MARCO (also known as SCARA2) gene (Gene ID: 8685) in human. MARCO is strongly expressed in macrophages of several tissues in humans with sepsis. It is a scavenger receptor that is readily upregulated following bacterial infection. Hence, it forms a back-up system through which the organism can quickly arm more cells with highly efficient phagocytosing receptors. MARCO is a single-pass type II membrane protein that binds both Gram-positive and Gram-negative bacteria via an extracellular, C-terminal, scavenger receptor cysteine-rich (SRCR) domain. The bacteria-binding region is localized immediately proximal to the cysteine-rich region of domain V. Human and mouse MARCO exhibit about 68% sequence identity. However, the human MARCO subunit chain differs from the mouse chain in that it does not have any cysteine residues outside the cysteine-rich domain V, whereas the mouse chain has one cysteine residue within the intracellular domain I and two in the extracellular spacer domain III. (Ref.: Elomaa, O., et al. (1998). J. Biol. Chem. 273(8): 4350-4538).
Spécificité
This polyclonal antibody detects macrophage receptor MARCO in RAW264.7 cells.
Immunogène
KLH-cojugated peptide corresponding to 12 amino acids from the C-terminal region of human MARCO.
Application
Anti-MARCO, Cat. No. ABN1389, is a highly specific rabbit polyclonal antibody that targets Macrophage receptor MARCO and has been tested in Western Blotting.
Research Category Neuroscience
Western Blotting Analysis: A 1:500 dilution from a representative lot detected MARCO in 10 µg of A549 cell lysate.
Qualité
Evaluated by Western Blotting in RAW264.7 cells.
Western Blotting Analysis (WB): A 1:5000 Dilution of this antibody detected Macrophage receptor MARCO in 10 µg RAW264.7 cells .
Description de la cible
~60 kDa observed; 52.65 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Forme physique
Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
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