ABE1354
Anti-Exo1
from rabbit
Synonyme(s) :
Exonuclease 1, hExo1, Exonuclease I, hExoI
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Espèces réactives
human
Conditionnement
antibody small pack of 25 μg
Technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... EXO1(9156)
Description générale
Exonuclease 1 (UniProt: Q9UQ84; also known as hExo1, Exonuclease I, hExoI) is encoded by the EXO1 (also known as EXOI, HEX1) gene (Gene ID: 9156) in human. Exonuclease 1 is a magnesium-dependent protein with 5′ to 3′ exonuclease and an RNase H activity. It may also possess a cryptic 3′→5′ double-stranded DNA exonuclease activity. It is highly expressed in bone marrow, testis and thymus. Higher levels of expression are also observed in fetal liver. Lower expression has been observed in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen, and stomach. It functions in DNA mismatch repair to excise mismatch-containing DNA tracts directed by strand breaks located either 5′ or 3′ to the mismatch. It interacts physically with the DNA mismatch repair proteins, Msh2 and Mlh1, and is involved in the excision of the mispaired nucleotide. Exonuclease 1 is phosphorylated upon DNA damage and in response to agents stalling DNA replication, probably by ATM or ATR. It is shown to be phosphorylated at Serine 454, Threonine 621, and Serine 714 upon DNA-damage caused by treatment with hydroxyurea, but not upon IR treatment. The hydroxyurea-induced triple phosphorylation of exonuclease 1 facilitates destabilization/degradation of the protein.
Spécificité
This rabbit polyclonal antibody detects Exonuclease 1 in human cells. It targets an epitope within 16 amino acids from the C-terminal half.
Immunogène
Epitope: unknown
KLH-conjugted linear peptide corresponding to 16 amino acids from the C-terminal half of human Exonuclease 1. The immunogen sequence is conserved in both isoforms 1 and 2.
Application
Anti-Exo1, Cat. No. ABE1354, is a highly specific rabbit polyclonal antibody that targets Exonuclease 1 and has been tested for use in Immunoprecipitation and Western Blotting.
Immunoprecipitation Analysis: A representative lot detected Exo1 in HeLa cell lysate (Courtesy of Dr. Zhongsheng You at Washington University in St. Louis).
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Exo1 in HeLa and siExo1 hela cell lysates (Courtesy of Dr. Zhongsheng You at Washington University in St. Louis).
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Exo1 in HeLa and siExo1 hela cell lysates (Courtesy of Dr. Zhongsheng You at Washington University in St. Louis).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Qualité
Evaluated by Western Blotting in 293T cells transfected with GFP-Exo1.
Western Blotting Analysis: 2 µg/mL of this antibody detected Exo1 in 293T cells transfected with GFP-Exo1.
Western Blotting Analysis: 2 µg/mL of this antibody detected Exo1 in 293T cells transfected with GFP-Exo1.
Description de la cible
~145 kDa observed; 94.10 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Forme physique
Affinity Purified
Format: Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Shan Li et al.
Molecular cell, 83(4), 556-573 (2023-01-26)
The protection of DNA replication forks under stress is essential for genome maintenance and cancer suppression. One mechanism of fork protection involves an elevation in intracellular Ca2+ ([Ca2+]i), which in turn activates CaMKK2 and AMPK to prevent uncontrolled fork processing
Xiao Wu et al.
Nature communications, 12(1), 4373-4373 (2021-07-18)
Although homologous recombination (HR) is indicated as a high-fidelity repair mechanism, break-induced replication (BIR), a subtype of HR, is a mutagenic mechanism that leads to chromosome rearrangements. It remains poorly understood how cells suppress mutagenic BIR. Trapping of Topoisomerase 1
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