560000

Sputolysin^® Reagent

Intended for the isolation of epithelial cells, pathogenic or saprophytic bacteria, fungi, and yeasts from sputum.

• Numéro CAS: 3483-12-3
• Numéro MDL: MFCD00004877
• Code UNSPSC : 12161703
• Nomenclature NACRES : NA.25

Propriétés

• Niveau de qualité: 100
• Forme: liquid
• Pertinence de la réaction: reagent type: reductant
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; protect from light
• Conditions d'expédition: ambient
• Température de stockage: 15-25°C
• InChI: 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m0/s1
• Clé InChI: VHJLVAABSRFDPM-IMJSIDKUSA-N

Description

Description générale

A concentrate of dithiothreitol (DTT) in phosphate buffer. Useful for the isolation of pathogenic or saprophytic bacteria, fungi, yeast, and epithelial cells from sputum and other mucoid fluids.

Concentrate of Dithiothreitol (DTT; Cat. No. 233155) in phosphate buffer, pH 6.5-7.5. Intended for the isolation of epithelial cells, pathogenic or saprophytic bacteria, fungi, and yeasts from sputum. Each vial is sufficient to make 100 ml of working solution.

Note: 1 set = 20 x 10 ml.

Avertissement

Toxicity: Harmful (C)

Forme physique

20 vials each containing 10 ml of concentrated DTT OR 1 vial containing 10 ml of concentrated DTT.

Notes préparatoires

Dilute the contents of one vial to 100 ml with sterile distilled water. Swirl gently to dissolve any crystals. If the diluted material appears cloudy, the solution may be filtered to remove any insoluble particles. The diluted solution is ~6.5 mM dithiothreitol in 100 mM phosphate buffer, pH 7.0.

Reconstitution

Once a vial is opened the unused concentrate should be purged with an inert gas (e.g. N₂) and refrigerated (4°C). Diluted solutions, stored aseptically under an inert gas, are stable for up to 48 h at 4°C.

Autres remarques

Hirsch, S.R., et al. 1969. J. Lab. Clin. Med.74, 346.
Shah, R.R. and Dye, W.E. 1966. Am. Rev. Respir. Dis.94, 454.
Dixon, J.M.S. and Miller, D.C. 1965 (November). Lancet p. 1046.
Cleland, W.W. 1964. Biochemistry3, 480.
May, J.R. 1953 (September). Lancet p. 535.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

SPUTOLYSIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H319
• Conseils de prudence: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Classification des risques: Eye Irrit. 2
• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable