492041

Nrf2 Activator II, AI-1

The Nrf2 Activator II, AI-1, also referenced under CAS 75483-04-4, modulates the biological activity of Nrf2. This small molecule/inhibitor is primarily used for Biochemicals applications.

• Synonyme(s) : Nrf2 Activator II, AI-1, Ethyl-4-chloro-1-methyl-2-oxo-1,2-dihydroquinoline-3-carboxylate
• Formule empirique (notation de Hill): C₁₃H₁₂ClNO₃
• Numéro CAS: 75483-04-4
• Poids moléculaire : 265.69
• Numéro MDL: MFCD00634787
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.51

Propriétés

• Niveau de qualité: 100
• Essai: ≥97% (HPLC)
• Forme: solid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated; protect from light
• Couleur: off-white
• Solubilité: DMSO: 50 mg/mL
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Chaîne SMILES: Clc1c2c([n]([c](c1C(=O)OCC)=O)C)cccc2
• InChI: 1S/C13H12ClNO3/c1-3-18-13(17)10-11(14)8-6-4-5-7-9(8)15(2)12(10)16/h4-7H,3H2,1-2H3
• Clé InChI: XUJQDJGGHFNYDY-UHFFFAOYSA-N

Description

Description générale

A cell-permeable chloro-quinolinone compound that selectively and covalently modifies Kelch-like ECH-associated protein 1 (Keap1)-Cys¹⁵¹ and represses Keap1 function and reduces Nrf2 (Nuclear factor erythroid 2-related factor 2)-Keap1 interaction. Shown to stabilize Nrf2 by inhibiting the Keap1-driven ubiquitination of Nrf2 and thereby activating the Nrf2-ARE (antioxidant response element) pathway (EC₅₀ = 2.7 µM in neuroblastoma IMR-32 cells). Offers protection against H₂O₂ induced oxidative stress, but unlike tert-butylhydroquinone. AI-1 does not act as a free radical scavenger. Its anti-oxidant effects are mediated via Nrf2 induced antioxidant response. Does not affect mutant ARE where GC are substituted with AT within the core ARE sequence.

Actions biochimiques/physiologiques

Cell permeable: yes

Primary Target
Keap1

Reversible: no

Conditionnement

Packaged under inert gas

Avertissement

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Use only fresh DMSO for reconstitution.

Autres remarques

Hur, W., et al. 2010. Chem. Biol.17, 537.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3