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Sigma-Aldrich

Anti-Dopamine D₁ Receptor Rabbit pAb

lyophilized, Calbiochem®

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Forme d'anticorps

serum

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

lyophilized

Ne contient pas

preservative

Espèces réactives

human, mouse, rat

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze

Isotype

IgG

Conditions d'expédition

ambient

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... DRD1(1812)
rat ... Drd1(24316)

Description générale

Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~50 kDa dopamine D1 receptor protein.
Recognizes the dopamine D1 receptor in rat brain. Does not cross-react with other dopamine receptors.
This Anti-Dopamine D₁ Receptor Antibody is validated for use in ELISA, WB, Frozen Sections, Free-Floating Sections, Paraffin Sections for the detection of Dopamine D₁ Receptor.

Immunogène

Human
a synthetic peptide (MDGTGLVVERDFSC) corresponding to amino acids 9-21 of human D₁dopamine receptor

Application

ELISA (see comments)

Immunoblotting (1:1000)

Frozen Sections (1:5000)

Free-Floating Sections (1:5000)

Paraffin Sections (not recommended)

Avertissement

Toxicity: Standard Handling (A)

Forme physique

Undiluted serum.

Reconstitution

Reconstitute the antibody with 100 µl PBS containing 1% BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).

Remarque sur l'analyse

Positive Control
Rat brain

Autres remarques

Mignini, F., et al. 2000. J. Autonom. Pharmacol.20, 325.
This antibody has also been reported to work in ELISA. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat′s milk. For frozen sections a 1:5000 dilution of the reconstituted serum demonstrates specific staining of rat brain sections including the medial septum, nucleus accumbens, dentate gyrus, globus pallidus, medial forebrain bundle, cortex regions 1-3, substantia nigra reticulata, and the ventral tegmental area. Strong nuclear staining of oligodendrocytes was also observed. Variables associated with assay conditions will dictate the proper working dilution.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Hye-Min Jeon et al.
Neoplasia (New York, N.Y.), 39, 100894-100894 (2023-03-28)
Recent studies indicate that signaling molecules traditionally associated with central nervous system function play critical roles in cancer. Dopamine receptor signaling is implicated in various cancers including glioblastoma (GBM) and it is a recognized therapeutic target, as evidenced by recent
Florian Giesert et al.
PloS one, 8(5), e63778-e63778 (2013-05-16)
Missense mutations in the leucine-rich repeat kinase 2 gene (LRRK2) are linked to autosomal dominant forms of Parkinson's disease (PD). In order to get insights into the physiological role of Lrrk2, we examined the distribution of Lrrk2 mRNA and different
Jacqueline S Coley et al.
PloS one, 10(2), e0117450-e0117450 (2015-02-04)
Drug abuse is a major comorbidity of HIV infection and cognitive disorders are often more severe in the drug abusing HIV infected population. CD14+CD16+ monocytes, a mature subpopulation of peripheral blood monocytes, are key mediators of HIV neuropathogenesis. Infected CD14+CD16+
Balaji Krishnan et al.
The European journal of neuroscience, 31(6), 1027-1042 (2010-04-10)
Corticotropin-releasing factor (CRF) in the amygdala is involved in stress responses. Moreover, dopaminergic neurotransmission in the brain reward system including the amygdala plays a significant role in the pathology of cocaine addiction. The present study analysed CRF-induced synaptic plasticity, its
Tina M Calderon et al.
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology, 12(2), 353-370 (2017-01-31)
In human immunodeficiency virus-1 (HIV) infected individuals, substance abuse may accelerate the development and/or increase the severity of HIV associated neurocognitive disorders (HAND). It is proposed that CD14+CD16+ monocytes mediate HIV entry into the central nervous system (CNS) and that

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