06-429-I

Anti-IGF-IRα subunit Antibody

from chicken, purified by affinity chromatography

• Synonyme(s) : Insulin-like growth factor 1 receptor, Insulin-like growth factor I receptor, IGF-I receptor, CD221, Insulin-like growth factor 1 receptor alpha chain, Insulin-like growth factor 1 receptor beta chain, IGF-IRα subunit
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: chicken
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): rhesus macaque (based on 100% sequence homology)
• Technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgY
• Numéro d'accès NCBI: NP_000866
• Numéro d'accès UniProt: P08069
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... IGF1R(3480)

Description

Description générale

Insulin-like growth factor 1 receptor (EC:2.7.10.1; UniProt P08069; also known as CD221, IGF-I receptor, Insulin-like growth factor I receptor) is encoded by the IGF1R (also known as CD221, IGFIR, IGFR, JTK13) gene (Gene ID 3480) in human. The insulin-like growth factor 1 (IGF-1) receptor has tyrosine kinase that is activated by IGF-1 and IGF-2 binding. IGF-1 receptor is initially produced as a precursor protein with an aignal peptide sequence (aa 1-30). Post-translational cleavages produce one extracellular alpha (aa 31-736) chain and one transmembrane beta chain (aa 741 – 1367) linked together by a disulfide bond. Two sets of alpha-beta dimers then form the functional tetrameric receptor complex via a disulfide linkage between their alpha chain. Upon ligand binding, the alpha chains induce the tyrosine auto-phosphorylation of the beta chains, which in turn triggers cell type-specific downstream signaling cascade of events.

Immunogène

Epitope: Third Fibronectin Type III (FnIII 3) domain

KLH-conjugated synthetic peptide [C-KYADGTIDIEEVTENPKT] corresponding to amino acid residues 672-689 of human IGF-IR alpha chain.

Application

Immunoprecipitation Analysis: 5.0 µg from a representative lot immunoprecipitated human IGF-IRα subunit in 0.5 mg of human IGF-IR-expressing C4 cell lysate.
Immunocytochemistry Analysis: 2.0 µg/mL from a representative lot detected IGF-IRα subunit in human IGF-IR-expressing C4 cells.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

This Anti-IGF-IRα subunit Antibody is validated for use in Western Blotting, Immunoprecipitation, Immunocytochemistry for the detection of IGF-IRα subunit.

Qualité

Evaluated by Western Blotting in human IGF-IR-expressing murine fibroblast LISN C4 cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected IGF-IRα subunit in 10 µg of human IGF-IR-expressing murine fibroblast LISN C4 cell lysate.

Description de la cible

~110/180 kDa observed

Forme physique

Affinity purified

Purified chicken polyclonal IgY antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable