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GERPN2209

ECL™ Western Blotting Detection Reagents

Cytiva RPN2209

Synonym(s):

ECL detection reagents, Western blot reagents

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About This Item

UNSPSC Code:
41105332
NACRES:
NA.32

feature

wetted part: no

packaging

pkg of 1 kit

manufacturer/tradename

Cytiva RPN2209

color

Detection reagent 1: Colorless Detection reagent 2: Colorless

storage temp.

2-8°C

General description

Amersham™ ECL™ Western Blotting Detection Reagents were the world′s first commercial chemiluminescent reagents for Western blotting and are still the most widely used. They are at least 10-fold more sensitive than colorimetric assays and produce excellent and reliable results every time.

These reagents use enhanced luminol-based detection which is suitable for all routine western blotting. ECL™ Western Blotting Detection Reagents in combination with Hybond® ECL™ membranes form a complete solution for Western blot applications.

Contents include the following:
GERPN2109, 62.5 mL each of ECL™ Detection Reagents 1 and 2 (1000 cm2)
GERPN2209, 125 mL each (2000 cm2)
GERPN2106, 250 mL each (4000 cm2)
GERPN2134, 375 mL each (6000 cm2)
Emission duration max. 2 h

Application

Western Blotting

Features and Benefits

  • High sensitivity, ~10 pg in model system.
  • Proven performance, the most frequently cited ECL™ Western Blotting Detection Reagents in publications
  • Detectable on Hyperfilm ECL™ or with CCD imager
  • Recommended mebrane Hybond ECL™

Components

Sufficient for detection of 2000 cm2 of membrane:
125 mL Detection reagent 1(color : colorless)
125 mL Detection reagent 2 (color: colorless)

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Amersham is a trademark of Cytiva
ECL is a trademark of Cytiva
Hybond is a registered trademark of Cytiva

Storage Class Code

12 - Non Combustible Liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Qinmiao Huang et al.
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Inflammation is fundamental in osteoarthritis (OA) pathogenesis. Semaphorin 4A (Sema4A) has been implicated in immune-associated diseases, however, its role in OA remains unclear. In this study, we show that Sema4A is upregulated in knee OA articular cartilage as well as
Ping Zhou et al.
International journal of biological sciences, 16(2), 204-215 (2020-01-14)
Chronic renal failure (CRF), also known as chronic kidney disease (CKD), is a common renal disorder characterized by gradual kidney dysfunction. Molecular dissection reveals that transforming growth factor beta (TGF-β) plays a central role in the pathogenesis of CRF. However
Gennaro Gambardella et al.
Science advances, 6(39) (2020-09-27)
Cells respond to starvation by shutting down protein synthesis and by activating catabolic processes, including autophagy, to recycle nutrients. This two-pronged response is mediated by the integrated stress response (ISR) through phosphorylation of eIF2α, which represses protein translation, and by

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