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T1706

Sigma-Aldrich

TransIT-CRISPR®

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.51

application(s)

CRISPR

shipped in

wet ice

storage temp.

−20°C

General description

Accomplish highly effective transfection of CRISPR/Cas9 DNA, RNA, Cas9-gRNA RNP (ribonucleoprotein) complexes and siRNA to knock-out, modify or knock-down genes of interest in a wide variety of cell types.

Features and Benefits

  • Highly efficient, non-liposomal polymeric transfection/delivery method for use with MISSION CRISPR/Cas9 DNA, RNA, Cas9 RNP Complexes and siRNA.
  • TransIT-CRISPR is a simple, fast, effective, and versatile. One transfection reagent to deliver CRISPR-Cas9 components in a variety of formats.
  • Effective in most cell lines including difficult-to-transfect cells.

Components

0.4ml and 1ml DNA and siRNA non-liposomal, polymeric transfection reagent

Linkage

To order MISSION® CRISPR reagents, please click here

For more information and transfection protocols, please visit SigmaAldrich.com/TransITCRISPR

Other Notes

  • TransIT-CRISPR has been tested in variety of cell types including U20S and HEK 293, A549 and HeLa among others.
  • It is recommended to transfect MISSION CRISPR DNA and RNA with the TransIT-CRISPR transfection reagent.
  • Sigma-Aldrich is the exclusive distributor of TransIT-CRISPR from Mirus Bio LLC.

Legal Information

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TransIT-CRISPR is a registered trademark of Mirus Bio LLC

Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Statements

H225,H319

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

57.2 °F - closed cup

Flash Point(C)

14.0 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Aitziber Buqué et al.
Nature communications, 11(1), 3819-3819 (2020-08-01)
Hormone receptor (HR)+ breast cancer (BC) causes most BC-related deaths, calling for improved therapeutic approaches. Despite expectations, immune checkpoint blockers (ICBs) are poorly active in patients with HR+ BC, in part reflecting the lack of preclinical models that recapitulate disease

Protocols

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PURedit™ CRISPR Synthetic Guide RNAs and Cas9 Protein

Guaranteed PURedit™ CRISPR synthetic gRNAs and Cas9 protein offer industry-leading on-site cutting and specificity

PURedit™ CRISPR Synthetic Guide RNAs and Cas9 Protein

Guaranteed PURedit™ CRISPR synthetic gRNAs and Cas9 protein offer industry-leading on-site cutting and specificity

PURedit™ CRISPR Synthetic Guide RNAs and Cas9 Protein

Guaranteed PURedit™ CRISPR synthetic gRNAs and Cas9 protein offer industry-leading on-site cutting and specificity

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