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LMO001

Sigma-Aldrich

MULTI-seq Lipid-Modified Oligos

for Single Cell and Single Nucleus Multiplexing

Synonym(s):

LMO, Lipid modified oligos

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About This Item

UNSPSC Code:
12352211
NACRES:
NA.51

Quality Level

200

form

liquid

usage

sufficient for 100 sample(s) (100RXN kit is adequate for labelling 100 samples when using 1 μL of each LMO per sample)
 sufficient for 30 sample(s) (30RXN kit is adequate for labelling 30 samples when using 1 μL of each LMO per sample)

concentration

50 μM (each)

technique(s)

multiplexing: suitable (Single Cell and Single Nucleus)

shipped in

dry ice

storage temp.

−20°C

General description

MULTI-seq, multiplexing using lipid-tagged indices (lipid-modified oligonucleotides), is a novel tool that facilitates multiplex single-cell and single-nucleus RNA sequencing. This technology provides sample barcoding that is compatible with any cell type or nucleus containing an accessible plasma membrane and allows for streamlined, pooled single-cell sample processing. Learn more about the experiments and data by reviewing our technical article – (MULTI-seq Sample Multiplexing for Single Cell Analysis and Sequencing)

Features and Benefits

  • Efficient. MULTI-seq increases the throughput and decreases the reagent cost of droplet-based single-cell RNA sequencing.
  • Flexible. Compatible with any droplet generation platform, the indexing system enables users to run and analyze up to 96 barcoded samples simultaneously in an 8-lane droplet device.
  • Clearer Results. The indices provide data quality advantages over non-indexed analysis methods in the form of doublet identification and retention of data from cells with low RNA content.

Components

The MULTI-seq Lipid Modified Oligos kit is comprised of a lignoceric amide-modified anchor DNA oligo solution and a palmitic amide-modified co-anchor DNA oligo solution. Together, these lipid-modified oligos embed into cell or nuclei membranes and provide a landing pad for DNA barcodes with a complementary 5’ sequence. The MULTI-seq Lipid Modified Oligos kit is intended for upstream sample preparation only, before pooling and single cell analysis.

Reagents Provided


  • LMO001A Lignoceric Anchor with DNA Oligo
  • LMO001B Palmitic Co-anchor with DNA Oligo
Each lipid-modified oligo is supplied at a concentration of 50 μM in water.

Other Notes

  • This product is for R&D use only. Not for drug, household, or other uses.
  • Unique barcode oligos are not included and must be purchased separately.
  • Barcode 3’ oligo design with poly-A tail for mRNA enrichment: 5′-CCTTGGCACCCGAGAATTCCA-8-base index-A30-3′
  • Barcode oligo design for 5’ cDNA library synthesis: 5’-CCTTGGCACCCGAGAATTCCA-8-base indexCCCATATAAGAAA-3’
  • In addition, 96 x 5′-end and 96 x 3′-end unique barcode oligos are available to purchase separately. Details on our custom barcode product, Next-Gen Sequencing Oligos (NGSO), can be found at SigmaAldrich.com/nextgenoligos. For minimal cross-contamination of barcode primers, order NGSO-Silver or preferably, NGSO-Gold quality. Please review the specifications available and then submit a quote request for the MULTI-seq barcodes to dnaoligos@milliporesigma.com. Sequences are not provided prior to purchase but will be included on product documentation at the time of delivery.

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Storage Class Code

12 - Non Combustible Liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Daniel V Brown et al.
Genomics, 116(2), 110793-110793 (2024-01-15)
Single-cell RNA sequencing (scRNA-Seq) has emerged as a powerful tool for understanding cellular heterogeneity and function. However the choice of sample multiplexing reagents can impact data quality and experimental outcomes. In this study, we compared various multiplexing reagents, including MULTI-Seq
Christopher S McGinnis et al.
Nature methods, 16(7), 619-626 (2019-06-19)
Sample multiplexing facilitates scRNA-seq by reducing costs and identifying artifacts such as cell doublets. However, universal and scalable sample barcoding strategies have not been described. We therefore developed MULTI-seq: multiplexing using lipid-tagged indices for single-cell and single-nucleus RNA sequencing. MULTI-seq
Henrik Gezelius et al.
NAR genomics and bioinformatics, 6(1), lqae001-lqae001 (2024-01-30)
Functional precision medicine (FPM) aims to optimize patient-specific drug selection based on the unique characteristics of their cancer cells. Recent advancements in high throughput ex vivo drug profiling have accelerated interest in FPM. Here, we present a proof-of-concept study for

Articles

MULTI-seq Sample Multiplexing for Single Cell Analysis and Sequencing

Use of MULTI-seq lipid-modified oligos, protocol, and troubleshooting guide for PCR Assays and Sequencing applications.

MULTI-seq Sample Multiplexing for Single Cell Analysis and Sequencing

Use of MULTI-seq lipid-modified oligos, protocol, and troubleshooting guide for PCR Assays and Sequencing applications.

MULTI-seq Sample Multiplexing for Single Cell Analysis and Sequencing

Use of MULTI-seq lipid-modified oligos, protocol, and troubleshooting guide for PCR Assays and Sequencing applications.

MULTI-seq Sample Multiplexing for Single Cell Analysis and Sequencing

Use of MULTI-seq lipid-modified oligos, protocol, and troubleshooting guide for PCR Assays and Sequencing applications.

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