A4737

Bovine Serum Albumin

lyophilized powder, protease, essentially free, ≥98% (agarose gel electrophoresis)

• Synonym(s): Albumin from bovine serum, Albumin bovine serum, BSA, Bovine albumin
• CAS Number: 9048-46-8
• EC Number: 232-936-2
• MDL number: MFCD00130384
• UNSPSC Code: 12352202

Properties

• biological source: bovine
• Assay: ≥98% (agarose gel electrophoresis)
• form: lyophilized powder
• mol wt: ~66 kDa
• packaging: poly bottle of
• origin: New Zealand origin
• technique(s): ligand binding assay: suitable
• impurities: ≤8.0% water (Karl Fischer)
• pH: 4.8-7.5
• solubility: H₂O: soluble 50 mg/mL
• UniProt accession no.: P02769
• foreign activity: protease, essentially free
• storage temp.: 2-8°C
• Gene Information: bovine ... ALB(280717)

Description

General description

The most abundant plasma protein in mammals is albumin. It is produced in the liver and transferred as a non-glycosylated protein into the plasma. This multifunctional protein can bind to ligands.

Application

Bovine serum albumin (BSA) is extensively used for drug delivery since it is widely available, cheap and easily purified, has ligand-binding properties, and is widely accepted in the pharmaceutical industry.

Biochem/physiol Actions

Albumin is the most important circulatory protein involved in the control of Ca²⁺ (and Mg²⁺) levels in mammals.

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Purified by chromatography

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Sourced and manufactured in New Zealand

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable