EMT6/VCR/R
96042323, mouse unknown/unspecified, Epithelial-like
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About This Item
UNSPSC Code:
41106514
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Product Name
EMT6/VCR/R, 96042323
biological source
mouse unknown/unspecified
packaging
tube of 5 μg 96042323-DNA-5UG
pkg of vial of cells 96042323-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse mammary, drug-resistant
Cell Line Description
EMT6/VCR/R is a drug-resistant variant of the mouse mammary tumour cell line EMT6/P (Sigma Catalogue number. 96042344). It was derived from the parent line by continuous growth in increasing concentrations of vincristine. EMT6/VCR/R was reported to be cross-resistant to other drugs of the multidrug-resistance (MDR) phenotype, showed reduced intracellular drug accumulation and hyperexpression of P-glycoprotein.
Application
Drug-resistance studies
Culture Medium
MEM(EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1μg/ml vincristine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37ºC. It is recommended to resusitate cells in drug-free medium. Add drug after growth is established.
Other Notes
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