JVM-2

96090515, human unknown/unspecified, Lymphoblastoid

• UNSPSC Code: 41106514

Properties

• product name: JVM-2, 96090515
• biological source: human unknown/unspecified
• growth mode: Suspension
• karyotype: 46,XX,-8,+t(3;8)(p12-13;q12),t(11;14)(q13;q32)
• morphology: Lymphoblastoid
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian B-prolymphocytic leukaemia, EBV-transformed

Cell Line Description

JVM-2 has been derived from EBV and TPA treated mononuclear cells from a 63 year old female patient with B-prolymphocytic leukaemia. Thirty-three percent of cells were shown to be positive for surface lambda light chain, 37% for IgA, 29% for IgG and 3% for IgM and IgD. In addition, 50% were found to express cytoplasmic IgM and lambda light chain. Expression of the following leukocyte differentiation antigens was found: CD9, CD19, CD20, CD23, CD24, MA6, CD37, CD38, FMC1, FMC7, MHC Class I, FMC16, MHC Class II DR/DP/DQ. The immunoglobulin heavy-and light-chain genes showed the same pattern of rearrangement as in the original prolymphocytes. The cells are lymphoblastoid with large floating aggregates present. The cell line has been eradicated from mycoplasma at ECACC.

Application

Ig expression of neoplastic cells

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11,13
D16S539: 12,13
D5S818: 11,12
D7S820: 10,11
THO1: 6,9
TPOX: 8,11
vWA: 17

Culture Medium

RPMI 1640 +2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9 x 100,000/ml; 5% CO₂; 37°C.

Other Notes

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