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  • Micro-emulsion synthesis, surface modification, and photophysical properties of Zn(1-x) Mn(x)S nanocrystals for biomolecular recognition.

Micro-emulsion synthesis, surface modification, and photophysical properties of Zn(1-x) Mn(x)S nanocrystals for biomolecular recognition.

IEEE transactions on nanobioscience (2012-12-06)
Elham Mohagheghpour, Fathollah Moztarzadeh, Mohammad Rabiee, Mohammadreza Tahriri, Maziar Ashuri, Hassan Sameie, Reza Salimi, Shahab Moghadas
RESUMEN

In this research, we mainly focused on the micro-emulsion synthesis of biotinylated ZnS (zinc sulfide) nanocrystals for avidin recognition. Various samples of Zn(1-x)Mn(x) S, with x = 0.0001, 0.007, 0.02, 0.03, 0.055, 0.09 and 0.13, prepared by quaternary W/O (water-in-oil) microemulsion system. Cyclohexane was used as oil, Triton X-100 as surfactant, n-hexanol as a co-surfactant and mercaptoethanol and thioglycolic acid as linking agents. The obtained products were evaluated by commonly techniques such as: scanning electron microscopy (SEM), transmission electron microscopy (TEM), zeta meter for measurement ZP (zeta potential) and fluorescence spectroscopy analyses. The above-experimental results indicated that the optimum doping concentration of Mn was ~ 5.5% . The fluorescence spectra of the doped crystals consist of orange-red emissions. Eventually, this research showed with increasing more than 18 μl biotin to nanocrystals, no changes were observed in the emission intensity spectra.

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Sigma-Aldrich
Triton X-100, for molecular biology
Sigma-Aldrich
2- Mercaptoetanol, for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)
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2- Mercaptoetanol, ≥99.0%
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Triton X-100 solution, BioUltra, for molecular biology, ~10% in H2O
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Thioglycolic acid, ≥99%
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2- Mercaptoetanol, BioUltra, for molecular biology, ≥99.0% (GC)
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1-Hexanol, reagent grade, 98%
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Sodium thioglycolate, ≥96.5% (iodometric)
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Triton X-100, BioXtra
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Thioglycolic acid, 98%
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1-Hexanol, anhydrous, ≥99%
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Triton X-45
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1-Hexanol, natural, ≥98%, FCC, FG
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Alcohol hexílico, FCC, FG
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Triton X-100, peroxide- and carbonyl-free
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Avidin from egg white, recombinant, expressed in corn, ≥12 units/mg protein (E1%/280)
Supelco
1-Hexanol, analytical standard
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Triton X-305 solution, 70% in H2O
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Triton X-102
Supelco
Thioglycolic acid solution, ~80% in H2O, for spectrophotometric det. of palladium, iron, uranium(VI), molybdates and nitrites
Sigma-Aldrich
1-Hexanol, ReagentPlus®, ≥99.5% (GC)
Octoxinol 10, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Thioglycolic acid solution, ~70 % (w/w) in H2O