- Methods for the determination of plasma or tissue glutathione levels.
Methods for the determination of plasma or tissue glutathione levels.
Methods in molecular biology (Clifton, N.J.) (2012-06-07)
Trent E Tipple, Lynette K Rogers
PMID22669674
RESUMEN
We present two different methods for determining levels of glutathione in complex biological samples and plasma. The DTNB/GR enzyme recycling method is sensitive and requires no specialized equipment. The HPLC method is particularly useful for situations in which sample amounts are limited. Detailed instructions for performing each method as well as the advantages and disadvantages of each are discussed in this chapter.
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Ácido 5,5′-ditiobis(2-nitrobenzoico), ≥98%, BioReagent, suitable for determination of sulfhydryl groups
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Cloruro de dansilo, BioReagent, suitable for amino acid labeling, powder and chunks, ≥99% (HPLC)
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ββ-Nicotinamida adenina dinucleótico 2′-fosfato reducido tetrasodium salt hydrate, ≥93% (HPLC)
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