ZRB2173

Anti-ACSL3 Antibody, clone 1I18 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Sinónimos: Fatty acid CoA ligase Acsl3;Arachidonate--CoA ligase;EC:6.2.1.15;Long-chain acyl-CoA synthetase 3;LACS 3;Long-chain-fatty-acid--CoA ligase 3;Medium-chain acyl-CoA ligase Acsl3
• Código UNSPSC: 12352203
• NACRES: NA.43

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 1I18, recombinant monoclonal
• descripción: 1I18 Clone
• Línea del producto: ZooMAb^® learn more
• formulario: lyophilized
• mol peso: calculated mol wt 80.42 kDa; observed mol wt ~75 kDa
• purificado por: using Protein A
• reactividad de especies: rat, human, mouse
• reactividad de especies (predicha por homología): porcine, monkey, bovine
• envase: antibody small pack of 25 μL
• características de los productos alternativos más sostenibles: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validación mejorada: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• técnicas: affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotipo: IgG
• secuencia del epítopo: C-terminal half
• Nº de acceso Protein ID: NP_976251
• Nº de acceso UniProt: O95573
• categoría alternativa más sostenible: , Aligned
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... ACSL3(2181)

Descripción

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Especificidad

Clone 1I18 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Fatty acid CoA ligase Acsl3 (ACSL3). It targets an epitope within 20 amino acids from the C-terminal half.

Inmunógeno

KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal half of human Fatty acid CoA ligase Acsl3 (ACSL3).

Aplicación

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected ACSL3 in HeLa cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected ACSL3 in A431 cell lysate and 1:1,000 dilution detected ACSL3 in C2C12, and C6 cell lysates.

Affinity Binding Assay: A representative lot of this antibody bound ACSL3 with a KD of 2.7 x 10-7 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ACSL3 in HeLa cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected ACSL3 in human placenta tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Descripción de destino

Fatty acid CoA ligase Acsl3 (UniProt: O95573; also known as Arachidonate--CoA ligase, EC:6.2.1.15, Long-chain acyl-CoA synthetase 3, LACS 3, Long-chain-fatty-acid--CoA ligase 3, Medium-chain acyl-CoA ligase Acsl3) is encoded by the ACSL3 (also known as ACS3, FACL3, LACS3) gene (Gene ID: 2181) in human. In mammals, Acyl-CoA synthetase (ACSL) enzymes are essential for de novo lipid synthesis, fatty acid catabolism, and remodeling of membranes. These ACSLs are divided into five sub-families based on the chain length of their preferred acyl groups. Activation of fatty acids requires a two-step reaction scheme catalyzed by ACSLs. In the first step, an acyl-AMP intermediate is formed from ATP. AMP is then exchanged with CoA to produce the activated acyl-CoA. ACSL3 is a single-pass type III membrane protein with a transmembrane domain (aa 21-41) and a long cytoplasmic domain )aa 42-720). It is mainly detected in the endoplasmic reticulum and peroxisomes. It is required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of very low-density lipoproteins (VLDL). It preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates. Overexpression of ACSL3 is shown in human lung cancer and is essential for mutant KRAS lung cancer tumorigenesis. Its suppression is linked to depletion of cellular ATP and death of lung cancer cells. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Padanad, MS., et al. (2016). Cell Reports 16(6); 1614-1628; Soupene, E., and Kuypers, FA. (2008). Exp. Biol. Med. (Maywood). 233(5): 507-521).

Forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitución

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1