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ZRB1567

Sigma-Aldrich

Anti-TLE3 Antibody, clone 1D15 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Sinónimos:

ESG3, Enhancer of split Groucho-like protein 3, Transducin-like enhancer protein 3

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25 μL
163,00 €
4 X 25 μL
335,00 €

163,00 €


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25 μL
163,00 €
4 X 25 μL
335,00 €

About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

163,00 €


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origen biológico

rabbit

Nivel de calidad

recombinante

expressed in HEK 293 cells

conjugado

unconjugated

forma del anticuerpo

purified antibody

tipo de anticuerpo

primary antibodies

clon

1D15, monoclonal
recombinant monoclonal

Línea del producto

ZooMAb® learn more

Formulario

lyophilized

mol peso

calculated mol wt 83.42 kDa
observed mol wt ~80 kDa

reactividad de especies

mouse, human

envase

antibody small pack of 25 μL

características de los productos alternativos más sostenibles

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

validación mejorada

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

técnicas

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotipo

IgG

Nº de acceso UniProt

categoría alternativa más sostenible

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... TLE3(7090)

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Especificidad

Clone 1D15 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Transducin-like enhancer protein 3 (TLE3). It targets an epitope within 21 amino acids from the N-terminal region.

Inmunógeno

KLH-conjugated linear peptide corresponding to 21 amino acids from the N-terminal region of human Transducin-like enhancer protein 3 (TLE3).

Aplicación

Anti-TLE3, clone 1D15 ZooMAb, Cat. No. ZRB1567, is a recombinant Rabbit monoclonal antibody that specifically targets TLE3 and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected TLE3 in HEK293 and NIH3T3 cell lysates.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TLE3 in HeLa, A431, HepG2, and NIH 3T3 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TLE3 in human vaginal tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Descripción de destino

Transducin-like enhancer protein 3 (UniProt: Q04726; also known as Enhancer of split Groucho-like protein 3, ESG3, TLE3) is encoded by the TLE3 (also known as KIAA1547) gene (Gene ID: 7090) in human. TLE3, a homotetrameric member of the Groucho/TLE family, is a transcriptional corepressor that binds to a number of transcription factors. Groucho/TLE proteins consist of a five-domain structures: a highly conserved Q domain that facilitates oligomerization of Gro/TLE molecules, a glycine/proline-rich (GP) domain that is essential for interaction of these proteins with histone deacetylases, a CcN domain that contains a nuclear localization sequence and CDC22 and casein kinase II phosphorylation sites, a serine/proline-rich domain, and a highly conserved WD40 domain that contains multiple tryptophan and aspartic acid tandem repeats. TLE3 is expressed in white adipose tissue and is shown to enhance the transcriptional activity of peroxisome proliferator-activated receptor (PPAR) and induce adipogenesis in preadipocytes. It also serves as a regulator of MyoD function during myogenesis. It interferes with MyoD function by disrupting the association between the basic helix-loop-helix domain of MyoD and E proteins. TLE3 is also shown to inhibit mitochondrial gene expression in beige adipocytes and conditional deletion of TLE3 in adipocytes is reported to promote mitochondrial oxidative metabolism and increase energy expenditure. It interacts with EBF2 and blocks its ability to promote the thermogenic transcriptional program. TLE3 is ubiquitinated by XIAP/BIRC4, but this ubiquitination does not affect its stability, nuclear localization, or capacity to tetramerize but inhibits its interaction with TCF7L2/TCF4. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Pearson, S., et al. (2019). Genes Dev. 2019. 33(13-14): 747-762; Kokabu, S., et al. (2017). J. Biol. Chem. J. Biol. Chem. 292(31); 12885-12894; Villanueva, CJ et al. (2011). Cell Metab. 13(4); 413-427).

Forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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