Upon creation of a cDNA library, quantitative qPCR or microarray analysis may be performed
WTA1
TransPlex® Whole Transcriptome Amplification Kit
DNA polymerase separate.
Sinónimos:
Transcriptome Amplification Kit
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| A ustedes/SKU | Disponibilidad | Precio |
|---|---|---|
50 reactions | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 3300,00 € |
Acerca de este artículo
NACRES:
NA.55
UNSPSC Code:
41121800
3300,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
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Permítanos ayudarleQuality Segment
technique(s)
whole genome amplification: suitable
dilution
(WTA)
input
purified DNA
shipped in
wet ice
storage temp.
−20°C
General description
TransPlex®, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3′-bias. Microgram quantities of amplification product generated from tissue, cultured cells, formalin-fixed samples, or serum are suitable for downstream applications such as qPCR and microarray analyses.
Application
TransPlex® Whole Transcriptome Amplification Kit has been used to synthesize double-stranded cDNA. It has also been used in the amplification of cDNA.[1]
Suitable for use with downstream applications including:
- qPCR
- microarray analysis
- cloning
Biochem/physiol Actions
The WTA process involves two steps. In the first step, sample RNA is reverse transcribed with non-self-complementary primers composed of a quasi-random 3′ end and a universal 5′ end. As polymerization proceeds, displaced single strands serve as new templates for primer annealing and extension. The resultant OmniPlex cDNA library, comprised of random, overlapping 100 - 1000 base fragments flanked by universal end sequence, is then amplified by PCR with the universal primer to produce WTA product.
Features and Benefits
- Amplification of total RNA in less than 4 hours with less than 30 minutes of "hands-on" time
- Only 5 ng of starting material required to produce a highly representative library from total RNA
- Microgram quantities of amplification product generated from intact RNA from tissue, cultured cells, serum, or degraded RNA from formalin-fixed paraffin-embedded samples
Legal Information
TransPlex is a registered trademark of Rubicon Genomics, Inc.
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Este artículo | |||
|---|---|---|---|
| technique(s) whole genome amplification: suitable | technique(s) whole genome amplification: suitable | technique(s) whole genome amplification: suitable | technique(s) whole genome amplification: suitable |
| dilution (WTA) | dilution (WTA) | dilution (WTA) | dilution (WGA) |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| input purified DNA | input purified RNA | input purified RNA | input purified DNA |
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
Clase de almacenamiento
12 - Non Combustible Liquids
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Can TransPlex® Whole Transcriptome Amplification Kit, Product WTA1, be used for downstream applications?
1 respuesta-
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What is the concentration of the dNTPs supplied with the TransPlex® Whole Transcriptome Amplification Kit, Product WTA1?
1 respuesta-
The dNTP Mix (D6193) is supplied as 10 mM each.
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What is the difference between WTA and WGA? I use the TransPlex® Whole Transcriptome Amplification Kit, Product WTA1.
1 respuesta-
The starting material for WTA is RNA, whereas for WGA it is genomic DNA. After the RNA is reverse-transcribed into the DNA complement by WTA, the OmniPlex library is formed. This library is in turn amplified by PCR.
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Can TransPlex® Whole Transcriptome Amplification Kit, Product WTA1, be used to amplify archival fixed tissue or degraded samples?
1 respuesta-
Yes, the WTA kit effectively amplifies degraded RNA, including formalin-fixed and paraffin-embedded samples.
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What is the Department of Transportation shipping information for this product?
1 respuesta-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is the TransPlex® Whole Transcriptome Amplification Kit, Product WTA1, also applicable for ribosomal RNA and/or small RNA amplification?
1 respuesta-
Using the kit as is, Ribosomal RNA does appear to amplify. However, we have not quantitated how well it is amplified.
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