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W0876

Sigma-Aldrich

White′s Basal Salt Mixture

powder, suitable for plant cell culture

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.72

formulario

powder

Nivel de calidad

200

técnicas

cell culture | plant: suitable

aplicaciones

agriculture

temp. de almacenamiento

2-8°C

Descripción general

White′s Basal Salt Mixture is widely used in plant tissue culture.

Aplicación

White′s Basal Salt Mixture has been used:
  • as a medium for the precultured spores of Prothalli of Adiantum capillus for the generation of protonemata
  • to promote germination and cotton cotyledon unfolding
  • as a solidified medium component for culturing A. capillus and as a component of selection medium for gametophyte selection after particle bombardment

Variante de la fórmula

With the macro- and micronutrients as described by White (1968).

Media Formulation

Palabra de señalización

Danger

Frases de peligro

H272,H318,H373,H411

Clasificaciones de peligro

Aquatic Chronic 2 - Eye Dam. 1 - Ox. Sol. 3 - STOT RE 2 Inhalation

Órganos de actuación

Brain

Código de clase de almacenamiento

5.1B - Oxidizing hazardous materials

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Hiroko Kawai-Toyooka et al.
Plant & cell physiology, 45(11), 1648-1657 (2004-12-03)
RNA interference (RNAi) has become a powerful tool for determining gene function and is used in a wide variety of organisms. Since it is necessary to generate double-stranded RNA (dsRNA) as an inducer for RNAi, preparation of RNAi-inducing constructs is
White's standard nutrient solution
SINGH M and Krikorian AD
Annals of Botany, 47, 133-139 (1981)
K Rajasekaran
Methods in molecular biology (Clifton, N.J.), 1902, 35-45 (2018-12-14)
Biolistic transformation of cotton (Gossypium hirsutum L.) meristems, isolated from mature seed, is detailed in this report. A commercially available, helium-driven biolistic device (Bio-Rad PDS1000/He ) was used to bombard gold particles coated with a marker gene (uidA or "GUS")
S J Sata et al.
Methods in cell science : an official journal of the Society for In Vitro Biology, 22(4), 299-304 (2001-09-11)
An efficient novel method of direct somatic embryogenesis from basal tissue of garlic clove was developed. The influence of plant growth regulators, basal medium and explant type on somatic embryo induction was examined. The best plant growth regulator combination was
D Parfitt et al.
Journal of experimental botany, 55(406), 2169-2177 (2004-09-14)
If, following an inductive treatment of 2 d of continuous darkness, shoot apices of Pharbitis nil are cultured 1 d later on White's medium supplemented with 2% sucrose, they cannot form carpels, but they can if they are cultured on
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Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.

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