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U7752

Urease from Canavalia ensiformis (Jack bean)

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1 vial
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107,00 €

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Número CAS:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-656-0
NACRES:
NA.54
MDL number:
Número CE:
Biological source:
Canavalia ensiformis

107,00 €


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biological source

Canavalia ensiformis

Quality Segment

form

solid

mol wt

hexamer 545 kDa, trimer ~272 kDa

contains

~20% dithiothreitol

suitability

suitable for marker for electrophoresis (non-denaturing PAGE)

storage temp.

−20°C

General description

Urease is a multi-subunit nickel-dependent metalloenzyme. It is found abundantly in fungi, yeast, plants, and bacteria. It belongs to the superfamily of amidohydrolases and phosphotriesterases. Urease is also found in leguminous seeds.
Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620

Application

Urease from Canavalia ensiformis (Jack bean) has been used:
  • as a component of the molecular mass standard in electrophoresis
  • as a component of the standard in size-exclusion chromatography
  • as a reference in native-polyacrylamide gel electrophoresis (PAGE)

Biochem/physiol Actions

Urease is an essential enzyme of the nitrogen cycle. It plays a role in degrading urea into ammonia and carbamate. Urease provides a source of nitrogen for growth to various organisms. It also plays a major role in seed germination and seed chemical defense.

Other Notes

Contains approx. 1 mg protein

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Este artículo
U0251U4002U1875
description

-

description

Type C-3, powder, ≥600,000 units/g solid

description

Type IX, powder, 50,000-100,000 units/g solid

description

Type III, glycerol solution, 500-800 units/mL

biological source

Canavalia ensiformis

biological source

Canavalia ensiformis

biological source

-

biological source

Canavalia ensiformis

form

solid

form

powder

form

powder

form

glycerol solution

suitability

suitable for marker for electrophoresis (non-denaturing PAGE)

suitability

-

suitability

-

suitability

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

mol wt

trimer ~272 kDa, hexamer 545 kDa

mol wt

~544620 Da

mol wt

~544620 Da

mol wt

~544620

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200


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Pictogramas

Health hazardExclamation mark

Palabra de advertencia

Danger

Códigos de peligro

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órganos diana

Respiratory system

Clase de almacenamiento

11 - Combustible Solids

WGK

WGK 1



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Preguntas

  1. Do you know the activity in U/mg of this urease preparation?

    1 respuesta
    1. This product is intended for use as a molecular weight marker in electrophoresis. The activity is not determined. Please see the link below to review other Urease products that offer a unit activity:
      https://www.sigmaaldrich.com/substance/ureasefromcanavaliaensiformisjackbean123459002135

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