The Elastic Shear modulus diagram was produced by employing a protocol that included mixing the polymer components. Subsequently, the elastic shear modulus of each hydrogel was measured at 23°C after 20 minutes using a Malvern Kinexus Pro rotational Rheometer, following the manufacturer’s protocol.
TRUE7
TrueGel3D Hydrogel Kits
SLO-DEXTRAN, allow cell recovery, CD cell-degradable crosslinker
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NACRES:
NA.71
UNSPSC Code:
12352207
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−70°C
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General description
The gel is formed by stable thioether bonds between thiol groups on CD cell-degradable crosslinker and thio-reactive groups on SLO-DEXTRAN. The polymer, SLO-DEXTRAN is functionalized with thiol-reactive groups which react with crosslinker at slower rate than maleimides in FAST-DEXTRAN. SLO-Dextran does not have sites for cell attachment, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1UM) prior to the crosslinking step.
The CD cell-degradable crosslinker is composed of matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala), which allows cells to spread and migrate by secreting matrix metalloproteases (MMP1, MMP3, MMP7 and MMP9).
TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.
The CD cell-degradable crosslinker is composed of matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala), which allows cells to spread and migrate by secreting matrix metalloproteases (MMP1, MMP3, MMP7 and MMP9).
TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.
Application
TRUE7-1KT is used to generate hydrogels of different stiffness (0.15 to 5 KPa shear modulus) to mimic in-vivo conditions and to investigate the effect of mechanical stimuli on cell physiology and behavior. The SLO-DEXTRAN based hydrogels are used in microchannels and also in syringes for injection. These hydrogels are also employed to study cell migration of neutrophils, dendritic cells and lymphocytes.
Features and Benefits
- Slow gelling (takes 2.5 minutes to 6 minutes, depends on gel stiffness)
- Flexibility in modulating gel stiffness
- Contains MMP-cleavable crosslinker
- Allow cell recovery and post-culture analyses
- Cell inert hydrogel (if not customized with RGD peptide)
Other Notes
TRUE7-1KT contains SLO-DEXTRAN solution in phosphate buffer and lyophilized CD cell-degradable crosslinker with reconstitution buffer and water.
Disclaimer
Avoid prolonged exposure of CD cell-degradable crosslinker to air.
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
wgk
WGK 3
Clase de almacenamiento
10 - Combustible liquids
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TrueGel3D™ is a synthetic hydrogels platform for 3D cell culture that aim to mimic the natural extracellular environment for more physiological culture conditions
Troubleshoot TrueGel3D™ hydrogel formation and cell viability issues with RGD-degradable polymer and CD cell-degradable crosslinker.
Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)
Protocolos
An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation
TrueGel3D™ is a chemically defined hydrogel for 3D cell growth, facilitating microchannel filling with slow gelation.
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Instructions
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What is the protocol used to produce the Elastic Shear modulus diagram mentioned in the document titled "Advanced Protocol: How to Customize your TrueGel3D Hydrogel"?
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