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T7902

Thermolysin from Geobacillus stearothermophilus

Name: Thermolysin from <I>Geobacillus stearothermophilus</I>
Brand: SIGMA

powder, BioReagent, 30-350 units/mg protein (E1%/280), suitable for cell culture

Sinónimos:

Protease from Geobacillus stearothermophilus, Thermophilic-bacterial protease

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Número CAS:

9073-78-3

UNSPSC Code:

12352204

NACRES:

NA.75

EC Number:

232-973-4

MDL number:

MFCD00165549

Número CE:

3.4.24.27 (BRENDA, IUBMB)

eCl@ss:

32160410

Specific activity:

30-350 units/mg protein (E1%/280)

Biological source:

Geobacillus stearothermophilus

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Propiedades

biological source

Geobacillus stearothermophilus

Quality Segment

200

product line

BioReagent

form

powder

specific activity

30-350 units/mg protein (E1%/280)

mol wt

34.6 kDa

purified by

crystallization

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

storage temp.

−20°C

Descripción

Application

A thermostable (thermophilic) extracellular metalloendopeptidase containing four calcium ions. Cofactors are zinc and calcium. Hydrolyzes protein bonds on the N-terminal side of hydrophobic amino acid residues. The pH optimum is 8.0 and the optimal temperature for activity is 70 °C. Considerably stable from pH 5 to 9.5. Thermolysin has a low cleavage specificity, therefore, it produces a number of short fragments that are suitable for sequencing. Preferential cleavage: X-cleavage-Y-Z where X=any amino acid; Y=Leu, Phe, Ile, Val, Met, Ala and Z is any amino acid other than Pro. Cleavage N-terminal to Leu is preferred over cleavage of N-terminal to Phe which is preferred over the others. Often used to do limited proteolysis for peptide mapping and studies of protein structure and conformational changes.

Physical form

lyophilized powder containing calcium and sodium acetate buffer salts

Other Notes

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

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Este artículo	P1512	C9722	T9935
Sigma-Aldrich T7902 Thermolysin from Geobacillus stearothermophilus	Sigma-Aldrich P1512 Thermolysin from Geobacillus stearothermophilus	Sigma-Aldrich C9722 Colagenasa from Clostridium histolyticum	Sigma-Aldrich T9935 Tripsina from bovine pancreas
description powder, BioReagent, 30-350 units/mg protein (E1%/280), suitable for cell culture	description Type X, lyophilized powder, 30-350 units/mg protein (E1%/280)	description lyophilized powder (from 0.2μm filtered solution), 0.5-5.0 FALGPA units/mg solid, suitable for cell culture	description essentially salt-free, lyophilized powder, ≥9,000 BAEE units/mg protein, BioReagent, suitable for cell culture
specific activity 30-350 units/mg protein (E1%/280)	specific activity 30-350 units/mg protein (E1%/280)	specific activity 0.5-5.0 FALGPA units/mg solid	specific activity ≥9,000 BAEE units/mg protein
biological source Geobacillus stearothermophilus	biological source Geobacillus stearothermophilus	biological source Clostridium histolyticum	biological source -
technique(s) cell culture \| mammalian: suitable, single cell analysis: suitable	technique(s) -	technique(s) cell culture \| mammalian: suitable, single cell analysis: suitable	technique(s) cell culture \| mammalian: suitable
form powder	form lyophilized powder	form lyophilized powder (from 0.2μm filtered solution)	form essentially salt-free, lyophilized powder
mol wt 34.6 kDa	mol wt 34.6 kDa by amino acid sequence	mol wt -	mol wt 23.8 kDa
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

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signalword

Danger

Clase de almacenamiento

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

target_organs

Respiratory system

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

wgk

WGK 1

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