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T5912

Sigma-Aldrich

Tris Buffered Saline

10X solution

Sinónimos:

TBS

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1 L
185,00 €

185,00 €


Disponible para envío el10 de abril de 2025Detalles


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1 L
185,00 €

About This Item

Número MDL:
Código UNSPSC:
12161700
NACRES:
NA.25

185,00 €


Disponible para envío el10 de abril de 2025Detalles


Solicitar un pedido a granel

Nombre del producto

Tris Buffered Saline, 10 ×, solution

esterilidad

0.2 μm filtered

Formulario

solution

concentración

10 ×

temp. de almacenamiento

2-8°C

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Aplicación

Tris Buffered Saline has been used as washing buffer for electrodes[1]. It was used to wash microtiter plates for western blot experiments and screening methods.[2]

Otras notas

Washing buffer for alkaline phosphatase or peroxidase conjugates in Western blotting. Working solution will contain 20 mM Tris, pH approx. 7.4, and 0.9% NaCl

Idoneidad

Suitable for use as a washing buffer for alkaline phosphatase or peroxidase conjugates in dot blot assay.

Producto relacionado

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Certificados de análisis (COA)

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Larry H Stanker et al.
Journal of immunological methods, 336(1), 1-8 (2008-05-03)
Botulinum neurotoxins (BoNT), produced by the anaerobic bacterium Clostridium botulinum, cause severe neuroparalytic disease and are considered the most toxic biological agents known. While botulism is rare in the U.S. it often is fatal if not treated quickly, and recovery
Rapid prototyping of multifunctional microfluidic cartridges for electrochemical biosensing platforms
Kin J
Sensors and Actuators B, Chemical, 202, 60-66 (2014)
Edward Greenfield et al.
eLife, 3 (2014-12-11)
The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of 50 papers in the field of cancer biology published between 2010 and 2012. This Registered Report describes the proposed replication plan
Jingtao Zhong et al.
International journal of molecular medicine, 41(5), 2893-2900 (2018-02-14)
Secretory clusterin (sCLU) is overexpressed in cancer and is associated with resistance to chemotherapy in several types of cancer, including hepatocellular carcinoma (HCC). Sorafenib (SOR), a multikinase inhibitor of Raf/mitogen‑activated protein kinase kinase/extracellular signal‑regulated kinase (ERK) signaling and the receptor
Nicholas Wheelhouse et al.
Scientific reports, 6, 37150-37150 (2016-11-15)
Waddlia chondrophila is a Gram-negative intracellular bacterial organism that is related to classical chlamydial species and has been implicated as a cause of abortion in cattle. Despite an increasing number of observational studies linking W. chondrophila infection to cattle abortion

Artículos

This solution is used as a substrate for detecting horseradish-peroxidase conjugates in Western blotting.

Preguntas

1–5 de 5 Preguntas  
  1. Which is the buffer composition? Thank you!

    1 respuesta
    1. The composition of this product consists of 20mM Tris and 0.9% NaCl at the 1X concentration.

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 respuesta
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. How can I determine the shelf life / expiration / retest date of this product?

    1 respuesta
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  4. I am interested in Tris Buffered Saline solution 10x (T5912) - I know this is sterile filtered but the specification sheet does not state "Sterile" or "pass" for sterility test. Do you provide a sterility test result on this product?

    1 respuesta
    1. While this material is sterile-filtered as a part of the production process, sterility testing is not performed on the finished product. This product is tested for Appearance, pH, Conductivity, and Dot Blot suitability. Please see the link below to review the product specifications:
      https://www.sigmaaldrich.com/specification-sheets/183/178/T5912-BULK________SIGMA____.pdf

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  5. What is the CAS number for T5912-1L?

    1 respuesta
    1. Tris Buffered Saline is not assigned a CAS (Chemical Abstract Service) number. This is a multi-component formulation. CAS numbers are assigned to individual compounds only.

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