STS0033

Agarose tablets

Low EEO, For molecular biology (200 x 500mg tablets)

• Sinónimos: 3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose LE, Agarose tablets
• Número de CAS: 9012-36-6
• Número MDL: MFCD00081294
• Código UNSPSC: 41105317
• NACRES: NB.21

Propiedades

• origen biológico: algae (marine)
• grado: for molecular biology
• Formulario: tablet
• impurezas: ≤10.0% moisture content
• color: white
• ELECTROENDÓSMOSIS (EEO): 0.09-0.13 (-Mr)
• temperatura de transición: transition temp 34.5-37.5 °C
• fuerza de gel: ≥1200 g/cm² (1% gel)
• trazas de anión: sulfate (SO₄^2-): ≤0.15%
• idoneidad: suitable for electrophoresis; suitable for molecular biology
• actividad extraña: DNase, RNase, none detected
• cadena SMILES: O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O
• InChI: 1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
• Clave InChI: MJQHZNBUODTQTK-WKGBVCLCSA-N

Descripción

Descripción general

Agarose is commonly used in research for gel electrophoresis to separate DNA and RNA fragments. It is a natural polysaccharide isolated and purified from red algae. It has a linear structure consisting of alternating D-galactose and 3,6-anhydro-L-galactose units.[1] When dissolved in solution, upon cooling, it forms a solid yet porous gel to use as a medium to separate nucleic acid fragments by size. When an electrical current is applied the charged molecules migrate through the gel.

Aplicación

Agarose tablets can be used in research applications for:

• Separation and analysis of nucleic acids by agarose gel electrophoresis, Northern Blot, or Southern Blot[2]
• Single Cell Gel Electrophoresis (SCGE), also known as comet assays
• Hydrocolloid gels to study the structural influence of gels on the release of carbohydrates[3]
• Ouchterlony double immunodiffusion plates used to quantify antibodies and antigens
• Radial immunodiffusion (RID)

Características y beneficios

• Qualified for molecular biology applications
• Free of endonucleases: DNase and RNase
• Convenient, quick and easy to use
• Low EEO and gelling temperature for routine analysis
• High gel strength 1,200 g/cm2 (1% gel)
• Separates DNA & RNA fragments 100bp - 23kb in 2% - 0.5% agarose gel, respectively.
• Northern or Southern blotting, preferred for fragment sizes >1kb

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable