Data is not available to indicate whether DNA contamination could affect sequencing. However, including DNase treatment is recommended to minimize genomic DNA in the sequencing reads.
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| A ustedes/SKU | Disponibilidad | Precio |
|---|---|---|
1 kit | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 1560,00 € |
Acerca de este artículo
1560,00 €
usage
sufficient for 250 purifications
Quality Segment
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
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technique(s)
RNA purification: suitable
test parameters
: ~30 min kit run time, sample volume: 100 mg
greener alternative category
storage temp.
15-25°C
General description
Después de moler el tejido hasta un polvo fino en nitrógeno líquido, se lisan las células, y los restos celulares se separan física y químicamente del ARN endógeno. A continuación el ARN se une a un sustrato de sílice sustentado en columna y varias etapas de lavado eliminan los contaminantes remanentes.
El ARN total es eluido de la columna y utilizado en las aplicaciones habituales como inmunotransferencias Northern, RT-PCR y qRT-PCR.
Application
- para extraer ARN de material molido en nitrógeno líquido
- para aislar el ARN total de las plántulas mutantes y wt (naturales)
- para extraer el ARN total de muestras de hojas
- para extraer ARN de todos los tejidos vegetales
Features and Benefits
- Produce hasta 60 μg de ARN concentrado puro por preparación
- Protocolo eficiente que permite la purificación del ARN en 30 minutos o menos
- Especialmente diseñado para la investigación con tejidos vegetales difíciles
Legal Information
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Este artículo | |||
|---|---|---|---|
| description sufficient for 250 purifications | description sufficient for 50 purifications | description sufficient for 350 purifications | description sufficient for 10 purifications |
| usage sufficient for 250 purifications | usage sufficient for 50 purifications | usage sufficient for 350 purifications | usage sufficient for 10 purifications |
| technique(s) RNA purification: suitable | technique(s) RNA purification: suitable | technique(s) RNA purification: suitable | technique(s) RNA purification: suitable |
| greener alternative category | greener alternative category | greener alternative category | greener alternative category , Aligned |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| test parameters : ~30 min kit run time, sample volume: 100 mg | test parameters : ~30 min kit run time, sample volume: 100 mg | test parameters sample volume: up to 10^7 cells or 40 mg of tissue, : <30 min kit run time | test parameters sample volume: 100 mg, : ~30 min kit run time |
| sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product |
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Solo componentes del kit
- Binding Solution
- Lysis Solution
- Wash Solution 1
Los componentes del kit también están disponibles por separado
- 2-Mercaptoethanol, Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration) 2
signalword
Danger
Hazard Classifications
Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral
target_organs
Liver,Heart
Clase de almacenamiento
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 3
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I’m using your plant RNA extraction kit for RNA-seq. If I skip the DNase treatment, could DNA contamination affect the sequencing library, or is it mandatory to include this step? Thank you.
1 respuesta-
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does it sufficient for small RNA and/or non-coding RNA extraction?
1 respuesta-
Yes, this kit is sufficient for small RNA. However, It is worth noting that small RNA molecules, such as microRNA, siRNA, tRNA, 5S rRNA, are not efficiently recovered using the standard procedure. If desired, these small RNA molecules can be recovered by increasing the amount of binding solution, as described in Protocol A under Procedure Step 4.
For more information, please see the protocol associated with this product:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/323/177/strn250bul.pdf¿Le ha resultado útil?
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