SRP6483

Carbonic Anhydrase 9/CA9 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Sinónimos: CA-IX, CAIX, G250, MN, P54/58N, pMW
• Código UNSPSC: 12352204
• NACRES: NA.32

Propiedades

• origen biológico: human
• recombinante: expressed in HEK 293 cells
• etiqueta: 6-His tagged (C-terminus)
• Análisis: ≥95% (SDS-PAGE)
• formulario: lyophilized
• mol peso: calculated mol wt 41.2 kDa; observed mol wt 53-54 kDa (DTT-reduced. Protein migrates due to glycosylation. Gln 38 is the predicted N-terminal.)
• envase: pkg of 10 μg
• impurezas: <1 EU/μg endotoxin (LAL test)
• Nº de acceso UniProt: Q16790
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... CA9(768)

Descripción

Descripción general

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes. CAs form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs rather slowly in the absence of a catalyst. One of the functions of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. The active site of most carbonic anhydrases contains a zinc ion. There are at least five distinct CA families (α, β, γ, δ and ε). Carbonic anhydrase 9 (CA9 / CAIX), also known as Membrane antigen MN (MN), Renal cell carcinoma-associated antigen G250, which belongs to the α-carbonic anhydrase family. CA9 / CAIX with an optimal activity at pH 6.49. Reversible hydration of carbon dioxide. CA IX participates in pH regulation. CA9 may be involved in the control of cell proliferation and transformation. CA-IX appears to be a novel specific biomarker for a cervical neoplasia.

Forma física

Lyophilized from 0.22 μm filtered solution in 20 mM MES and 100 mM NaCl, pH 6.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitución

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable