SRP6082

TRXB from Escherichia coli

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• Sinónimos: TRXR, Thioredoxin reductase
• Código UNSPSC: 12352200
• NACRES: NA.32

Propiedades

• origen biológico: Escherichia coli
• recombinante: expressed in E. coli
• Ensayo: ≥90% (SDS-PAGE)
• Formulario: liquid
• mol peso: 34.6 kDa
• envase: pkg of 100 μg
• Nº de acceso NCBI: P0A9P4
• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C
• Información sobre el gen: Escherichia coli ... TRXB(949054)

Descripción

Descripción general

Thioredoxin reductase (TrxR) is encoded by TRXB gene. TrxR is one of the member of flavoprotein family of pyridine nucleotide-disulphide oxidoreductases, which also includes lipoamide dehydrogenase, glutathione reductase and mercuric ion reductase. Escherichia coli TrxR is a 70-kDa homodimeric flavoprotein, each monomer of this protein contains a FAD prosthetic group, an NADPH domain and an active site with redox-active disulphide. The catalytic site (-Cys-Ala-Thr-Cys-) in Escherichia coli TrxB is localized on the NADPH domain, whereas in humans TrxB catalytic site (-Cys-Val-Asn-Val-Gly-Cys-) is part of the FAD domain.[1][2]

Acciones bioquímicas o fisiológicas

Thioredoxin reductase (TRXB) catalyzes the reduction of oxidized thioredoxin using nicotinamide adenine dinucleotide phosphate (NADPH).[1][3] In thioredoxin pathway, this reduced thioredoxin reduce disulfide bonds in proteins localized in the cytoplasm and is implicated in the recycling of an essential enzyme ribonucleotide reductase. TrxR is essentially involved in protection against oxidation stress, cell growth and transformation, and the recycling of ascorbate from its oxidized form.[1]

Forma física

1 mg/mL solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol and 1mM DTT.

Nota de preparación

Centrifuge the vial prior to opening.

Otras notas

MGTTKHSKLL ILGSGPAGYT AAVYAARANL QPVLITGMEK GGQLTTTTEV ENWPGDPNDL TGPLLMERMH EHATKFETEI IFDHINKVDL QNRPFRLNGD NGEYTCDALI IATGASARYL GLPSEEAFKG RGVSACATCD GFFYRNQKVA VIGGGNTAVE EALYLSNIAS EVHLIHRRDG FRAEKILIKR LMDKVENGNI ILHTNRTLEE VTGDQMGVTG VRLRDTQNSD NIESLDVAGL FVAIGHSPNT AIFEGQLELE NGYIKVQSGI HGNATQTSIP GVFAAGDVMD HIYRQAITSA GTGCMAALDA ERYLDGLADA K.

Información de seguridad

• Pictogramas: GHS07
• Palabra de señalización: Warning
• Frases de peligro: H319
• Consejos de prudencia: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Clasificaciones de peligro: Eye Irrit. 2
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable