SRP6045

Glucokinase from human liver

recombinant, expressed in E. coli, ≥77% (SDS-PAGE)

• Sinónimos: Hexokinase, Hexokinase, liver, Liver glucokinase
• Número de CAS: 9001-51-8
• Código UNSPSC: 12352204
• NACRES: NA.32

Propiedades

• origen biológico: human liver
• recombinante: expressed in E. coli
• Análisis: ≥77% (SDS-PAGE)
• formulario: liquid
• mol peso: 53.4 kDa
• envase: pkg of 10 μg
• concentración: ~1 mg/mL
• impurezas: Endotoxin, tested
• Nº de acceso NCBI: P35557
• Nº de acceso UniProt: P35557
• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C
• Información sobre el gen: human ... GCK(2645)

Descripción

Descripción general

The gene encoding glucokinase (GCK) is alternatively spliced to generate three different forms of the enzyme; one found in the pancreas and two found in the liver. The enzyme is expressed as a monomer and possesses one glucose-binding site. The gene encoding GCK is localized on human chromosome 7. Recombinant human liver glucokinase (Accession# NP_277042)) has a C-terminal FLAG tag and has 474 amino acid residues.

Acciones bioquímicas o fisiológicas

Glucose is phosphorylated to glucose-6-phosphate by glucokinases. The main function of glucokinase (GCK) is to regulate carbohydrate metabolism. The enzyme triggers the release of insulin in β-cells of pancreas. It can be useful for studies including enzyme kinetics, activator screening and kinase selectivity. Mutation in the gene encoding this protein is associated with a rare form of diabetes and the protein may also have a role in type 2 diabetes.

Definición de unidad

One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30°C. Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2 mM ATP, 25 mM glucose, 100 μg/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human liver glucokinase at 30°C for 30 min.

Forma física

50 mM potassium phosphate pH 7.4, 50 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, and 2.5% glycerol.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable