SRP0243

aRAF Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

• Sinónimos: A-Raf proto-oncogene, PKS2, v-Raf murine sarcoma 3611 viral oncogene homolog
• Código UNSPSC: 12352202
• NACRES: NA.32

Propiedades

• origen biológico: human
• recombinante: expressed in baculovirus infected insect cells
• Ensayo: ≥80% (SDS-PAGE)
• Formulario: aqueous solution
• mol peso: 63.6 kDa
• envase: pkg of 10 μg
• condiciones de almacenamiento: avoid repeated freeze/thaw cycles
• concentración: >0.02 mg/mL
• Nº de acceso NCBI: NM_001654
• Nº de acceso UniProt: P10398
• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C
• Información sobre el gen: human ... ARAF(369)

Descripción

Descripción general

Human A-RAF (GenBank Accession No. NM_001654), amino acids 282-end, containing the mutations YY301-302DD, MW = 63.6 kDa, with N-terminal GST tag, expressed in an Baculovirus infected Sf9 cell expression system.

Aplicación

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Forma física

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.

Nota de preparación

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.