SRP0211

PTP-D2/PTPN14 Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• Sinónimos: Cytoskeletal-associated protein tyrosine phosphatase, PTP36, PTPN14, Pez, Protein tyrosine phosphatase, non-receptor type 14
• Código UNSPSC: 12352200
• NACRES: NA.32

Propiedades

• origen biológico: human
• recombinante: expressed in E. coli
• Ensayo: ≥90% (SDS-PAGE)
• Formulario: aqueous solution
• mol peso: 61 kDa
• envase: pkg of 20 μg
• condiciones de almacenamiento: avoid repeated freeze/thaw cycles
• concentración: >0.02 mg/mL
• Nº de acceso NCBI: NM_005401
• Nº de acceso UniProt: Q15678
• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C
• Información sobre el gen: human ... PTPN14(5784)

Descripción

Descripción general

Catalytic domain, (amino acids 817-1188), (Gene bank accession number NM_005401) with N-terminal GST tag, MW = 61 kDa, expressed in an E. coli expression system.

Aplicación

Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.

Definición de unidad

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Forma física

Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.

Nota de preparación

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.