Complex of: Human EZH1, GenBank Accession No. NM_001991, amino acids 2-end, with N-terminal His tag, MW = 86 kDa. Human EED, NM_003797, amino acids with N-terminal DDDDK tag (FLAG), MW = 51 kDa. Human SUZ12,. NM_015355, amino acids 2-end, with N-terminal His tag.
Aplicación
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Definición de unidad
One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.
Forma física
Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide and 10% glycerol.
Nota de preparación
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
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