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SHC005V

MISSION® eGFP Control Transduction Particles

shRNA sequence targeting eGFP

Sinónimos:

MISSION®, MISSION® Control Transduction Particles

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1 pkg
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380,00 €

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NACRES:
NA.51
UNSPSC Code:
41106609
Concentration:
≥1x106 VP/ml (via p24 assay)
Technique(s):
capture ELISA: 106 TU/mL using p24

380,00 €


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Quality Segment

product line

MISSION®

concentration

≥1x106 VP/ml (via p24 assay)

technique(s)

capture ELISA: 106 TU/mL using p24

shipped in

dry ice

storage temp.

−70°C

General description

When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.
The MISSION eGFP shRNA Control Transduction Particles contain an shRNA sequence targeting eGFP (GenBank Accession No. pEGFP U55761). The eGFP shRNA Control Particles are useful as a positive knockdown control in experiments using cell lines expressing eGFP.
The eGFP shRNA Control Transduction Particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-eGFP shRNA (Prod. No. SHC005). Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the control transduction particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.

Application

To see more application data, protocols, vector maps visit sigma.com/shrna.

Legal Information

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

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Este artículo
SHC004VSHC002HSHC007V
description

shRNA sequence targeting eGFP

description

shRNA sequence targeting tGFP

description

Targets no known mammalian genes

description

shRNA sequence targeting luciferase

product line

MISSION®

product line

MISSION®

product line

MISSION®

product line

MISSION®

concentration

≥1x106 VP/ml (via p24 assay)

concentration

≥1x106 VP/ml (via p24 assay)

concentration

≥1x109 VP/ml (via p24 assay)

concentration

≥1x106 VP/ml (via p24 assay)

technique(s)

capture ELISA: 106 TU/mL using p24

technique(s)

capture ELISA: 106 TU/mL using p24

technique(s)

capture ELISA: 109 TU/mL using p24

technique(s)

capture ELISA: 106 TU/mL using p24

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

200


Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)



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