SEP050
Seppro® Supermix
LC2
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About This Item
Código UNSPSC:
12352200
NACRES:
NA.32
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lecho volumen
2 mL
columna P × L
6.4 mm × 63 mm
capacidad
loading(Flow Through volume from IgY 14 LC5 (SEP030))
temp. de almacenamiento
2-8°C
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Descripción general
Immunoaffinity fractionation of highly-abundant proteins (HAP) has proven to be one of the most effective approaches for overcoming the wide dynamic range of protein concentration, as well as enabling the detection of low abundant proteins (LAP). Avian polyclonal IgY (Immunoglobulin Yolk) antibodies have unique and advantageous features that allow for highly-specific fractionation of a complex protein mixture. IgY12 column can effectively remove 12 HAP from plasma. The next level of abundant protein, moderately abundant proteins (MAP), becomes an obstacle to access LAP, where the majority of biologically interesting and clinically important biomarkers reside. Therefore, isolation of MAP is a new challenge for effectively and accurately detecting and analyzing LAP.
To tackle this challenge, we further developed our IgY columns system by establishing a SuperMix column to separate MAP from LAP. The SuperMix System was developed by immunizing chickens with a flow-through fraction of IgY12 or IgY14 column and constructing the column with affinity-purified IgY antibodies against the flow-through proteins of IgY12 or IgY14.
To tackle this challenge, we further developed our IgY columns system by establishing a SuperMix column to separate MAP from LAP. The SuperMix System was developed by immunizing chickens with a flow-through fraction of IgY12 or IgY14 column and constructing the column with affinity-purified IgY antibodies against the flow-through proteins of IgY12 or IgY14.
Aplicación
Seppro® Supermix, LC2 (2mL column) is used to separate moderately abundant proteins (MAP) from human serum low abundant proteins (LAP) after specific human serum highly-abundant proteins (HAP) have been removed using Seppro® IgY12 or IgY14 affinity separation.
Otras notas
for removal of moderately abundant proteins from human serum or plasma
Almacenamiento y estabilidad
Store the column at 2-8 °C. After use, equilibrate the column with 1′ Dilution Buffer containing 0.02% sodium azide and store the column at 2-8 °C with the end-caps tightly sealed. Do Not Freeze the column.
Información legal
Seppro is a registered trademark of Merck KGaA, Darmstadt, Germany
Los componentes del kit también están disponibles por separado
Referencia del producto
Descripción
SDS
Código de clase de almacenamiento
10 - Combustible liquids
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Effect of plasma protein depletion on BNP-32 recovery.
Adam M Hawkridge et al.
Clinical chemistry, 54(5), 933-934 (2008-04-30)
Does plasma protein depletion increase lung liquid conductance?
P Dodek
American journal of respiratory and critical care medicine, 154(4 Pt 1), 1213-1215 (1996-10-01)
Majlinda Kullolli et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 939, 10-16 (2013-10-05)
Human plasma is a commonly used diagnostic fluid in clinical chemistry. In-depth plasma proteomic analysis is performed to search for disease biomarkers, however the large dynamic range of protein abundance in plasma presents a substantial analytical challenge. Removal of abundant
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