SAE0091

Lisostafina from Staphylococcus staphylolyticus

free of DNA contaminants, ≥500 units/mg protein, lyophilized powder, suitable for Microbiome research

• Sinónimos: Endopeptidasa glicil-glicina
• Número CAS: 9011-93-2
• UNSPSC Code: 12352202
• NACRES: NA.54
• Número CE: 3.4.24.75 (BRENDA, IUBMB)
• MDL number: MFCD00131556

Propiedades

• Nombre del producto: Lisostafina from Staphylococcus staphylolyticus, free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥500 units/mg protein
• biological source: microbial (Staphylococcus staphylotyticus)
• form: lyophilized powder
• specific activity: ≥500 units/mg protein
• mol wt: 27 kDa
• feature: DNA free
• input: bacteria and yeast sample(s)
• shipped in: wet ice
• storage temp.: −20°C

Descripción

Application

Purified extracellular lysostaphin from S. staphylolyticus SAE0091 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Lysostaphin is a zinc metalloenzyme isolated from a bacterial culture of Staphylococcus staphylolyticus. It has specific lytic action against other Staphylococcus species, including S. aureus.1,2 Lysostaphin has
hexosaminidase, amidase, and endopeptidase activities. It cleaves polyglycine crosslinks in the cellular wall which leads to cell lysis of Staphylococcus species, but not of other bacterial genera. [Pharmaceuticals 2010, 3(4), 1139-1161]
Lysostaphin is a single polypeptide chain of 246 amino acids, a molecular mass of 26,926 Da, isoelectric point of 9.5, (5) and an activity pH optimum of 7.5.(6)

Biochem/physiol Actions

La lisostafina es una endopeptidasa de cinc con un peso molecular de aproximadamente 25 kDa. Debido a que la lisostafina rompe los enlaces transversales de la poliglicina de la capa de peptidoglucanos de la pared celular de las especies de Staphylococcus , se ha encontrado útil para la lisis celular y también como potencial tratamiento antimicrobiano.
pH de actividad óptimo: ~7,5

Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic. pH Optimum for activity: ~7.5.

Physical form

Supplied as a lyophilized powder containing 50–70% protein with the balance primarily as NaCl.

Other Notes

One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37°C in a 6.0 ml reaction mixture.

Información de seguridad

• Pictogramas: GHS08
• Palabra de advertencia: Danger
• Códigos de peligro: H334
• Indicaciones de precaución: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Clase de almacenamiento: 11 - Combustible Solids
• ¿Qué está pasando?: WGK 3
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable