SAB4200729

Anti-Vinculin Antibody

mouse monoclonal, VIN-11-5

• Sinónimos: Anti-MV, Anti-Metavinculin, Anti-VINC1
• Código UNSPSC: 12352203
• NACRES: NA.41
• clon: VIN-11-5, monoclonal
• application: IF; IHC; IP
• reactividad de especies: human, monkey, rat, mouse, guinea pig, bovine, hamster, chicken, dog
• técnicas: immunoblotting: 0.125-0.25 μg/mL using mouse myoblast C2C12 cell extract; immunofluorescence: 5-10 μg/mL; immunohistochemistry: suitable; immunoprecipitation (IP): suitable
• citations: 16

Propiedades

• Nombre del producto: Anti-Vinculin antibody, Mouse monoclonal, clone VIN-11-5, purified from hybridoma cell culture
• origen biológico: mouse
• Nivel de calidad: 200
• forma del anticuerpo: purified from hybridoma cell culture
• tipo de anticuerpo: primary antibodies
• clon: VIN-11-5, monoclonal
• Formulario: buffered aqueous solution
• mol peso: ~120 kDa
• reactividad de especies: human, monkey, rat, mouse, guinea pig, bovine, hamster, chicken, dog
• concentración: ~1.0 mg/mL
• técnicas: immunoblotting: 0.125-0.25 μg/mL using mouse myoblast C2C12 cell extract; immunofluorescence: 5-10 μg/mL; immunohistochemistry: suitable; immunoprecipitation (IP): suitable
• isotipo: IgG1
• Nº de acceso UniProt: P18206
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: mouse ... Vcl(22330)

Descripción

Descripción general

Anti-Vinculin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the VIN-11-5 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with smooth muscle vinculin from chicken gizzard. Vinculin, also known as metavinculin is highly conserved scaffolding protein localized to focal adhesions and adherens junctions. Vinculin is a 116 kDa protein with 1066 aminoacids. Vinculin comprises eight anti-parallel α-helical bundles organized into five distinct domains. Domains 1-3 are arranged into a tri-lobar head with a molecular mass of 95 kDa and diameter of 80Å . The vinculin head binds many proteins including talin, IpaA, β-catenin, α-catenin and α-actinin. A 61 amino acid, proline-rich linker region (residues 837-878) connects the head and tail. The linker binds vasodilator-stimulated phosphoprotein (VASP), vinexin, ponsin and Arp2/3. The vinculin tail (amino acids 879-1066) is a 30 kDa helical bundle containing five helices connected by short loops (3-8 residues). The tail contains binding sites for vinculin head domain, paxillin, acidic phospholipids and actin. Within the cell vinculin exists either as open, active form or as a closed, auto-inhibited state. The vinculin gene is mapped to human chromosome 10q22.2. Metavinculin (150 kDa), a splice variant of vinculin, is co-expressed with vinculin in muscle tissues. These proteins are localized to the cell membrane, the I-band in the sarcomere and to the intercalated discs.

Inmunógeno

smooth muscle vinculin from chicken gizzard

Aplicación

Monoclonal Anti-Vinculin is recommended to use in various immunochemical assays, including immunoblot (~120kDa), immunofluorescence, immunohistochemistry and immunoprecipitation.

Acciones bioquímicas o fisiológicas

Vinculin is a cytoplasmic actin binding protein. It plays a key role in cell to cell and cell to matrix adhesion. It mediates adhesion by directly binding to F-actin and subsequently causing actin polymerization and binding of actin remodelling proteins. At high concentrations, Vinculin can undergo oligomerization and may be involved in the transmembrane assembly of adhesion plaques. Vinculin has been implicated in the control of adhesion, cell morphology and motility and muscle functions. Mutations in vinculin, affects the binding of vinculin to F-actin and impairs cell migration. It is a susceptible gene for dilated and hypertrophic cardiomyopathy. Mutations in vinculin may lead to sudden unexplained nocturnal death syndrome (SUNDS). In relation to cell adhesion and motility function, Vinculin is considered to have a crucial effect on tumor cells ability to invade tissues and hence to metastasize. Thus, it is suggested to be implicated in diagnosis of cancer progression and metastasis prognosis.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable