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SAB4200686

Sigma-Aldrich

Anti-Collagen, Type VII antibody, Mouse monoclonal

clone LH7.2, purified from hybridoma cell culture

Sinónimos:

Monoclonal Anti-Collagen, Type VII antibody produced in mouse, COL7A1, EBD1, EBDCT, EBR1, alpha 1, collagen, type VII

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

200

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

LH7.2, monoclonal

formulario

buffered aqueous solution

reactividad de especies

human

envase

antibody small pack of 25 μL

concentración

~1 mg/mL

técnicas

immunoblotting: suitable
immunohistochemistry: 1.5-3 μg/mL using frozen human tonsil sections

isotipo

IgG1

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... COL7A1(1294)

Categorías relacionadas

Descripción general

Monoclonal Anti-Collagen Type VII (mouse IgG1 isotype) is derived from the hybridoma LH7.2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Collagen Type VII (known also as Col7), which belongs to the collagen superfamily, is a major extracellular matrix component of the anchoring fibrils in lamina densa. COL7A1 (collagen type VII alpha 1 chain) gene codes for type VII collagen. It is located on human chromosome 3p. It is expressed by keratinocytes and fibroblasts. COL7A1 is the important component the anchoring fibrils.

Inmunógeno

insoluble fractions prepared from neonatal foreskin epidermal cells

Aplicación

Monoclonal Anti-Collagen, Type VII antibody produced in mouse has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunohistochemistry
  • immunofluorescence

Acciones bioquímicas o fisiológicas

Collagen type VII is very essential for adhesive connection between the dermis and the epidermal basement membrane zone (BMZ). Mutations in collagen type VII cause dystrophic forms of epidermolysis bullosa (including recessive dystrophic epidermolysis bullosa (RDEB)), which manifest as skin fragility and malformed enamel.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Genetic linkage of type VII collagen (COL7A1) to dominant dystrophic epidermolysis bullosa in families with abnormal anchoring fibrils.
Ryynaanen M, et al.
The Journal of Clinical Investigation (1992)
Julia Niskanen et al.
PloS one, 12(5), e0177527-e0177527 (2017-05-12)
A rare hereditary mechanobullous disorder called epidermolysis bullosa (EB) causes blistering in the skin and the mucosal membranes. To date, nineteen EB-related genes have been discovered in human and other species. We describe here a novel EB variant in dogs.
Nonsense variant in COL7A1 causes recessive dystrophic epidermolysis bullosa in Central Asian Shepherd dogs
Niskanen J, et al.
PLoS ONE, 12(5), e0177527-e0177527 (2017)
Deletion of a Pathogenic Mutation-Containing Exon of COL7A1 Allows Clonal Gene Editing Correction of RDEB Patient Epidermal Stem Cells.
Mencia A, et al.
Molecular Therapy. Nucleic Acids, 11, 68-78 (2018)
Dystrophic epidermolysis bullosa: COL7A1 mutation landscape in a multi-ethnic cohort of 152 extended families with high degree of customary consanguineous marriages.
Vahidnezhad H, et al.
The Journal of Investigative Dermatology, 137(3), 660-669 (2017)

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