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MAB1330

Sigma-Aldrich

Anti-Collagen Type II Antibody, clone COLL-II

ascites fluid, clone COLL-II, Chemicon®

Sinónimos:

Anti-ANFH, Anti-AOM, Anti-COL11A3, Anti-SEDC, Anti-STL1

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
clon:
COLL-II, monoclonal
application:
ELISA
IHC
reactividad de especies:
sheep, human, bovine, rat, pig, mouse, canine
técnicas:
ELISA: suitable
immunohistochemistry: suitable
citations:
36

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

ascites fluid

tipo de anticuerpo

primary antibodies

clon

COLL-II, monoclonal

reactividad de especies

sheep, human, bovine, rat, pig, mouse, canine

fabricante / nombre comercial

Chemicon®

técnicas

ELISA: suitable
immunohistochemistry: suitable

isotipo

IgG1

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

dry ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... COL2A1(1280)

Especificidad

Reacts with both pepsin-solubilized and CNBr-cleaved human and bovine collagen type II. No cross-reactivity with collagen types I, III, V and IX.

Inmunógeno

Human (cartilage specific) CNBr-cleaved collagen II.

Aplicación

Immunofluorescence: 1:2-1:5 with fresh, frozen or acetone-fixed material. Mild pepsin digestion is recommended to enhance staining.

Western Blot: 1:1000-1:2000 with chemiluminescent detection

ELISA at 1:100-1:500, pepsin treated samples.





Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins
This Anti-Collagen Type II Antibody, clone COLL-II is validated for use in ELISA, IH for the detection of Collagen Type II.

Forma física

Ascites fluid containing no preservatives.
Unpurified

Almacenamiento y estabilidad

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Nota de análisis

Control
Cartilage in lung or fetus

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Feng-Cheng Liu et al.
Nutrients, 11(5) (2019-05-30)
Genistein is an isoflavone extracted from soybean (Glycine max). This compound has anti-inflammatory, anti-oxidative, and anti-cancer effects; however, the mechanism underlying the effects of genistein on IL-1β-stimulated human osteoarthritis (OA) chondrocytes remains unknown. Our objectives in this study were to
Biwei Deng et al.
International journal of nanomedicine, 13, 8217-8230 (2018-12-18)
The osteoinductive behaviors of nitinol (NiTi)-based metal implants for bone regeneration are largely dependent on their surface composition and topology. Continuous-mode laser sintering often results in complete melting of the materials and aggregation of particles, which lack control of heat
L A Vonk et al.
Osteoarthritis and cartilage, 22(1), 145-153 (2013-11-26)
Hsa-miR-148a expression is decreased in Osteoarthritis (OA) cartilage, but its functional role in cartilage has never been studied. Therefore, our aim was to investigate the effects of overexpressing hsa-miR-148a on cartilage metabolism of OA chondrocytes. OA chondrocytes were transfected with
Xin Lu et al.
International journal of molecular medicine, 37(2), 509-516 (2015-12-29)
The aim of the present study was to examine the expression levels and role of hsa-miR-15a in osteoarthritis (OA), as well as the associated mechanisms. The expression levels of hsa-miR-15a and A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type
Yang-Jun Lao et al.
Orthopaedic surgery, 10(1), 56-63 (2018-02-13)
To investigate the effect of accumulated spinal axial biomechanical loading on mice lumbar disc and the feasibility of applying this method to establish a mice intervertebral disc degeneration model using a custom-made hot plate cage. In previous studies, we observed

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