S2647

Spectinomycin dihydrochloride pentahydrate

powder, γ-irradiated

• Fórmula empírica (notación de Hill): C₁₄H₂₄N₂O₇ · 2HCl · 5H₂O
• Número CAS: 22189-32-8
• Peso molecular: 495.35
• NACRES: NA.85
• PubChem Substance ID: 24899532
• UNSPSC Code: 12352200
• EC Number: 244-554-3
• MDL number: MFCD00150886

Propiedades

• biological source: synthetic (inorganic)
• Quality Segment: 200
• grade: Molecular Biology
• sterility: γ-irradiated
• form: powder
• potency: ≥500 μg per mg
• storage condition: (Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.)
• color: white to off-white
• solubility: H₂O: soluble at
• antibiotic activity spectrum: Gram-negative bacteria, Gram-positive bacteria
• mode of action: protein synthesis | interferes
• storage temp.: 2-8°C
• suitability: nonselective for Escherichia coli, nonselective for coliforms
• SMILES string: O.O.O.O.O.Cl.Cl.CN[C@@H]1[C@H](O)[C@H](NC)[C@H]2O[C@]3(O)[C@@H](O[C@H](C)CC3=O)O[C@@H]2[C@H]1O
• InChI: 1S/C14H24N2O7.2ClH.5H2O/c1-5-4-6(17)14(20)13(21-5)22-12-10(19)7(15-2)9(18)8(16-3)11(12)23-14;;;;;;;/h5,7-13,15-16,18-20H,4H2,1-3H3;2*1H;5*1H2/t5-,7-,8+,9+,10+,11-,12-,13+,14+;;;;;;;/m1......./s1
• InChI key: DCHJOVNPPSBWHK-UXXUFHFZSA-N

Descripción

General description

Chemical structure: aminoglycoside

Application

Spectinomycin is an aminocyclitol antibiotic derived from Streptomyces spectabilis. It has been shown to have a wide variety of uses, including treating acute gonorrheal urethritis and cervictis, to mark cell layers to monitor cell fate during leaf development, as a selection marker in plant related transformation systems for plant cells containing the marker gene Spcr, to study respiratory tract infections of cattle caused by Pasteurella multocida and Mannheimia haemolytica, and to generate plants deficient for the plastid-encoded RNA polymerase on MS-agar media. Spectinomycin also has use in amplification of low copy number plasmid carrying replicons. It is recommended for use in cell culture applications at 7.5-20 mg/L.

Biochem/physiol Actions

Mode of Action: Spectinomycin acts by inhibiting protein synthesis and elongation by binding to the bacterial 30S ribosomal subunit and interfering with peptidyl tRNA translocation.

Mode of Resistance: A mutation in rpsE, the gene for ribosomal protein S5, prevents the binding of spectinomycin. Resistance is also conferred by the aminoglycoside-3′-adenyltransferase gene (aadA).

Antimicrobial spectrum: Spectinomycin acts against gram-negative and gram-positive bacteria.

Packaging

100MG

Preparation Note

Stock solutions should be prepared at 10 mg/mL in water and filter sterilized.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.

Disclaimer

Solutions can be stored at 2-8°C for several weeks or at -20°C for more extended periods.

Información de seguridad

• Pictogramas: GHS07
• Palabra de advertencia: Warning
• Códigos de peligro: H315,H319,H335
• Indicaciones de precaución: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Órganos diana: Respiratory system
• Clase de almacenamiento: 11 - Combustible Solids
• WGK: WGK 2
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable
• EPI (equipos de protección individual): Eyeshields, Gloves, type N95 (US)