RDP200

GenElute^™ FFPE RNA/DNA Purification Plus Kit

• Código UNSPSC: 41105600
• NACRES: NA.52

Propiedades

• características de los productos alternativos más sostenibles: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• categoría alternativa más sostenible: Aligned,
• Condiciones de envío: wet ice
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

Sigma′s GenElute^™ FFPE RNA/DNA Purification Plus Kit provides a rapid method for the sequential isolation and purification of total RNA (including microRNA) and genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the nucleic acids over time. Sigma′s GenElute^™ FFPE RNA/DNA Purification Plus Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of nucleic acids. The kit is able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as the degree of fragmentation of the RNA will increase over time. The RNA is purified from other cellular components without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays. The purified genomic DNA is also of the highest quality, and can be used in PCR reactions, sequencing, Southern blotting and SNP analysis.

• Fast and easy processing using rapid spin-column format
• High yields and Quality of Nucleic Acids
• Separate fractionation of RNA and DNA
• Isolate total RNA, from large rRNA down to microRNA (miRNA)
• No phenol or chloroform extractions

Aplicación

RNA

• Bioanalyzer
• Quantitative, real-time RT-PCR for both large mRNA and small RNA including miRNA
• RT-PCR for both large mRNA and small RNA including miRNA
• Northern blotting
• RNase protection
• Primer extension
• Expression array assays
• Next Generation Sequencing
• microRNA Cloning
• miRNA array
• RNA-Seq
• Nanostring
• Fluidigm
• Droplet/Digital PCR

Genomic DNA

• PCR and Quantitative PCR
• Sequencing
• DNA Methylation Studies
• Southern blotting
• Microarrays
• SNP analysis

Características y beneficios

• Complete column purification - Separate fractionation of RNA and DNA
• Reduce variability - RNA and DNA are isolated from a single sample with no splitting of the lysate, thus reducing inconsistent results and variability
• Isolate a diversity of RNA species - All sizes of RNA are isolated, from large mRNA down to microRNA
• Isolate high quality RNA and DNA - The purified RNA and DNA are of the highest quality and can be used in a number of downstream applications

Definición de unidad

Preps

Información legal

GenElute is a trademark of Sigma-Aldrich Co. LLC

Información de seguridad

• Pictogramas: GHS08,GHS05,GHS07
• Palabra de señalización: Danger
• Frases de peligro: H302 + H332,H314,H334,H412
• Consejos de prudencia: P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Clasificaciones de peligro: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1C
• Riesgos supl.: EUH032
• Código de clase de almacenamiento: 8A - Combustible corrosive hazardous materials