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RAB1156

Sigma-Aldrich

Rat TIMP-2 ELISA Kit

for cell culture supernatants, plasma, and serum samples

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About This Item

Código UNSPSC:
41116158
NACRES:
NA.32

reactividad de especies

rat

envase

kit of 96 wells (12 strips x 8 wells)

técnicas

ELISA: suitable

entrada

sample type cell culture supernatant(s)
sample type serum sample(s)
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 20 pg/ml

método de detección

colorimetric

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

Información sobre el gen

Descripción general

Tissue inhibitor of metalloproteinase 2 (TIMP -2) is expressed in the brain especially in spinal motor neurons, cerebellar Purkinje cells, cerebral cortical, and hippocampal pyramidal cells. It is an inhibitor of matrix metalloproteinase and tumor angiogenesis. TIMP-2 is involved in matrix remodeling and stimulates quiescent cell proliferation. This ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of a target protein in biological samples, such as serum, plasma, cell culture supernatants, urine, and/or cell and tissue lysates.

Aplicación

For research use only. Not for use in diagnostic procedures.
Please refer to the attached Protocolfor details.
Rat TIMP-2 ELISA Kit has been used in enzyme-linked immunosorbent assay to quantify tissue inhibitor of metalloproteinase 2 (TIMP -2) in U87MG human glioblastoma cells.

Otras notas

A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number.

Pictogramas

Corrosion

Palabra de señalización

Warning

Frases de peligro

Consejos de prudencia

Clasificaciones de peligro

Met. Corr. 1

Código de clase de almacenamiento

8A - Combustible corrosive hazardous materials

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Listados normativos

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EU REACH Annex XVII (Restriction List)


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Diane M Jaworski et al.
Journal of neurochemistry, 98(1), 234-247 (2006-06-30)
Neuronal differentiation requires exquisitely timed cell cycle arrest for progenitors to acquire an appropriate neuronal cell fate and is achieved by communication between soluble signals, such as growth factors and extracellular matrix molecules. Here we report that the expression of
Dong-Wan Seo et al.
Cell, 114(2), 171-180 (2003-07-31)
Tissue inhibitors of metalloproteinases (TIMPs) suppress matrix metalloproteinase (MMP) activity critical for extracellular matrix turnover associated with both physiologic and pathologic tissue remodeling. We demonstrate here that TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo
Dimitra Bourboulia et al.
Oncotarget, 4(1), 166-176 (2013-02-02)
Tissue Inhibitor of Metalloproteinase 2 (TIMP-2) plays an essential role in regulating matrix remodeling, cell growth, differentiation, angiogenesis and apoptosis in vitro and in vivo. We have recently shown that TIMP-2-mediated inhibition of tumor growth is independent of matrix metalloproteinase-mediated
Shan Wu et al.
International journal of molecular medicine, 42(6), 3386-3394 (2018-10-03)
The present study investigated the underlying molecular mechanism by which Buthus martensii Karsch chlorotoxin (BmK CT) inhibits the invasion and metastasis of glioma cells and the possibility of 131I‑labeled BmK CT (131I‑BmK CT) as a novel targeted agent for the treatment of glioma. The

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