RAB0500

Human p53 ELISA Kit

for serum, plasma, cell culture supernatant and urine

• NACRES: NA.32
• UNSPSC Code: 41116158
• Gene information: human ... TP53(7157)
• Input: sample type cell culture supernatant(s); sample type urine; sample type serum; sample type plasma
• Species reactivity: human
• Storage temp.: −20°C
• Shipped in: wet ice

Propiedades

• species reactivity: human
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable, capture ELISA: suitable
• input: sample type cell culture supernatant(s); sample type urine; sample type serum; sample type plasma
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 0.3 ng/mL; standard curve range: 0.328-80 ng/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... TP53(7157)

Descripción

General description

Cellular tumor antigen p53 is a tumor-suppressor protein that comprises transactivation at N-terminus, central sequence-specific DNA-binding domain, and regulatory domain at C-terminus. It plays a key role in autophagy regulation. It inhibits tumors and acts as a cellular stress sensor especially during nutrient deprivation, hypoxia, and ribosome dysfunction. The Human p-53 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human p-53 in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human p53

Application

Human p53 ELISA Kit has been used for quantifying p53 using enzyme-linked immunosorbent assay (ELISA) in human myeloma RPMI-8226 cells, human colorectal cancer HCT-116 cells, human breast cancer MCF-7 cells and human leukemiaHL-60 cells.

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Los componentes del kit también están disponibles por separado

• RABELADB

  ELISA 5X Assay/Sample Diluent Buffer B (Item E1)

  SDS
• RABELADD

  ELISA 5X Assay/Sample Diluent Buffer D (Item K)

  SDS
• RABSTOP3

  ELISA Stop Solution (Item I)

  SDS
• RABTMB3

  ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  SDS
• RABWASH4

  20X Wash Buffer (Item B)

  SDS

Información de seguridad

• pictograms: GHS05
• signalword: Warning
• hcodes: H290
• pcodes: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Clase de almacenamiento: 10 - Combustible liquids