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RAB0369

Human MMP-7 ELISA Kit

on for cell culture supernatant

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1 kit
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539,00 €

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NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
human ... MMP7(4316)
Input:
sample type cell culture supernatant(s)
Species reactivity:
human
Storage temp.:
−20°C
Shipped in:
wet ice

539,00 €

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species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 150 pg/mL
standard curve range: 0.137-100 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... MMP7(4316)

General description

The human MMP-7 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MMP-7 in cell culture supernatants. Matrix metalloproteinase-7 (MMP-7)/matrilysin is a small proteolytic enzyme. It secretes zinc- and calcium-dependent endopeptidase. It is expressed in normal endometrial, bronchial, ductal, skin glandular epithelial, urogenital, and gastrointestinal tissues. MMP-7 plays a key role in cell proliferation, migration, invasion, and apoptosis. Elevated expression of MMP-7 is seen in several cancers, such as esophageal, gastric, gallbladder, pancreatic, colorectal, bladder, ovarian, breast, lung, and melanoma.

Immunogen

Recombinant Human MMP7

Application

Human MMP-7 ELISA Kit has been used in ELISA to measure the recovery of the cancer biomarkers/MMP-7 in isothermally vitrified samples. It has also been used in ELISA to determine MMP7 concentrations in multiple myeloma (MM) cell culture supernatants.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

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Human MMP-7 ELISA Kit on for cell culture supernatant

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Human MMP-1 ELISA Kit for cell and tissue lysates

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RAB0362

Human MMP-1 ELISA Kit

description

on for cell culture supernatant

description

for serum, plasma, conditioned medium, urine

description

for serum, plasma, cell culture supernatant and urine

description

for cell and tissue lysates

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

Gene Information

human ... MMP7(4316)

Gene Information

human ... MMP8(4317)

Gene Information

human ... MMP3(185250)

Gene Information

human ... MMP1(4312)

input

sample type cell culture supernatant(s)

input

sample type plasma
sample type serum
sample type urine
sample type conditioned medium

input

sample type cell culture supernatant(s)
sample type urine
sample type serum
sample type plasma

input

sample type tissue lysate
sample type cell lysate

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 150 pg/mL
standard curve range: 0.137-100 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 6 pg/mL
standard curve range: 8.23-6000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 300 pg/mL
standard curve range: 0.41-100 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 8 pg/mL
standard curve range: 24.69-18000 pg/mL

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

capture ELISA: suitable

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS & Precios
  • RABELADE
    ELISA 5X Assay/Sample Diluent Buffer E (Item E2)
    SDS
  • RABSTOP3
    ELISA Stop Solution (Item I)
    SDS
  • RABTMB3
    ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
    SDS
  • RABWASH4
    20X Wash Buffer (Item B)
    SDS

pictograms

Corrosion
GHS05

signalword

Warning

hcodes

H290

Hazard Classifications

Met. Corr. 1

Clase de almacenamiento

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documentos section.

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Preguntas

  1. Is the MMP7 a total form or an active form in this kit?

    1 respuesta

    1. The capture antibody used in this kit will recognize both the pro and active form of MMP-7. The immunogen is human recombinant MMP-7, aa18 - 267. The standard provided in the kit is the pro form,

      Response from Ray Bio - not to be included in response
      Capture Ab: Mouse Monoclonal IgG2B
      Immunogen: Mouse myeloma cell line NS0-derived recombinant human MMP 7
      aa18-aa267, accession# NP_002414.1
      Specificity: detects human MMP-7 in pro and active forms (seen in WB)

      Detection Ab: Polyclonal Goat IgG
      Immunogen: Mouse myeloma cell line NS0-derived recombinant human MMP 7
      aa18-aa267, accession # P09237

      Additionally, the standard used is recombinant Human MMP-7 in pro form (aa18-267).

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