R5636
Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae)
buffered aqueous solution
Sinónimos:
Transfer RNA, tRNA
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About This Item
Productos recomendados
grado
for molecular biology
Nivel de calidad
formulario
buffered aqueous solution
concentración
≥9 mg/mL
actividad extraña
DNase, Nickase, none detected
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
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Descripción general
Transfer RNA (tRNA) is isolated from baker′s yeast by phenol-chloroform extraction and ethanol precipitation. tRNAs are approximately 80 nucleotides long RNA molecules with a cloverleaf shaped secondary structure and a tertiary L-shaped structure.
Aplicación
Suitable for use as a carrier in nucleic acid purification and precipitation.
Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) has been used:
Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) has been used:
- in carrier solution as a part of control used for reverse transcriptase – quantitative polymerase reaction (RT-qPCR) assay
- as a component of prehybridization(4) and hybridization buffer in single-label in situ hybridization
- as a carrier to enhance recovery of RNA from small numbers of cells
Acciones bioquímicas o fisiológicas
Transfer RNAs (tRNAs) play an important role in translation. It is responsible for the addition of amino acids to ribosome for peptide chain formation. The tRNA contains an anticodon region for mRNA base pairing and two attachment regions: for amino acid binding and tRNA synthetase recognition. The ribosomal RNA facilitates the movement of tRNA along the mRNA.
Componentes
tRNA is provided in a solution in 10 mM Tris HCl (pH 7.4) in 1 mM EDTA.
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Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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