R4018
Ribonucleic acid, transfer, phenylalanine specific from brewer′s yeast
Sinónimos:
Transfer RNA, Yeast Phe tRNA, tRNA
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About This Item
Productos recomendados
grado
for molecular biology
formulario
lyophilized powder
mol peso
25 kDa
solubilidad
water: 2 mg/mL
temp. de almacenamiento
2-8°C
Descripción general
Transfer RNA is isolated from brewer′s yeast. This transfer RNA is specific for transfer of the amino acid phenylalanine.
The phenylalanine acceptor activity of 100% pure tRNA is 1,800 pmoles per A260 unit. Comparison of this number to lot-specific analysis may be used as an indication of product purity. Methods to further purify tRNA have been published.
The assay for this product is based on a published method. The activity is measured using a crude preparation of phenylalanyl transfer ribonucleic acid synthetase. The phenylalanine specific tRNA is used as a substrate for the enzyme in the assay.
This product is not suitable for use as carrier tRNA to aid in the ethanol precipitation of very small amounts of DNA. Product Nos. R 8508 and R 5636 are recommended for use as carrier tRNA for DNA purification and precipitation.
The phenylalanine acceptor activity of 100% pure tRNA is 1,800 pmoles per A260 unit. Comparison of this number to lot-specific analysis may be used as an indication of product purity. Methods to further purify tRNA have been published.
The assay for this product is based on a published method. The activity is measured using a crude preparation of phenylalanyl transfer ribonucleic acid synthetase. The phenylalanine specific tRNA is used as a substrate for the enzyme in the assay.
This product is not suitable for use as carrier tRNA to aid in the ethanol precipitation of very small amounts of DNA. Product Nos. R 8508 and R 5636 are recommended for use as carrier tRNA for DNA purification and precipitation.
Aplicación
Suitable for use:
- As a tRNA standard
- As a substrate in tRNA synthatase assays
- As a substrate in other reactions involving tRNAs
Componentes
tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml.
Nota de preparación
Prepared by method of Wimmer, E., et al., Biochemistry, 7, 2623 (1968).
Producto relacionado
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
13 - Non Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Mariana Pavon-Eternod et al.
Nucleic acids research, 37(21), 7268-7280 (2009-09-29)
Increased proliferation and elevated levels of protein synthesis are characteristics of transformed and tumor cells. Though components of the translation machinery are often misregulated in cancers, what role tRNA plays in cancer cells has not been explored. We compare genome-wide
W Yan et al.
The EMBO journal, 17(16), 4809-4817 (1998-08-26)
Correct folding of newly synthesized polypeptides is thought to be facilitated by Hsp70 molecular chaperones in conjunction with DnaJ cohort proteins. In Saccharomyces cerevisiae, SSB proteins are ribosome-associated Hsp70s which interact with the newly synthesized nascent polypeptide chain. Here we
Tissue-specific differences in human transfer RNA expression.
Dittmar, K.A., et al.
PLoS Genetics, 2, 2107-2107 (2006)
Noa Aharon-Hefetz et al.
eLife, 9 (2020-12-29)
Different subsets of the tRNA pool in human cells are expressed in different cellular conditions. The 'proliferation-tRNAs' are induced upon normal and cancerous cell division, while the 'differentiation-tRNAs' are active in non-dividing, differentiated cells. Here we examine the essentiality of
Andrey Chursov et al.
Nucleic acids research, 41(Web Server issue), W486-W491 (2013-06-15)
Although multiple biological phenomena are related to temperature (e.g. elevation of body temperature due to an illness, adaptation to environmental temperature conditions, biology of coldblooded versus warm-blooded organisms), the molecular mechanisms of these processes remain to be understood. Perturbations of
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