R4030

Anti-hnRNP-E1 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Sinónimos: Anti-HNRPX, Anti-PCBP1, Anti-hnRNP-X, Anti-poly(rC) binding protein 1
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• conjugado: unconjugated
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• Formulario: buffered aqueous solution
• mol peso: antigen ~37 kDa
• reactividad de especies: mouse, human, rat
• concentración: ~1.0 mg/mL
• técnicas: immunoprecipitation (IP): 5-10 μg using K562 cell extract; western blot: 0.5-1 μg/mL using K562 cell extract
• Nº de acceso UniProt: Q15365
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... PCBP1(5093) ; mouse ... Pcbp1(23983) ; rat ... Pcbp1(500242)

Descripción

Descripción general

Heterogeneous nuclear ribonucleoprotein E1 (hnRNP E1)/poly(rC)-binding protein 1 (PCBP1) has five major isoforms, hnRNP-E1-E4 and hnRNP-E2-KL (a splice variant of hnRNP-E2). It is a member of the PCBP family or the triple K homology (KH) domain containing protein family. All isoforms of hnRNPE contain three repeats of the ribonucleoprotein KH domain, designated KH1, KH2 and KH3. hnRNP-E1 and hnRNP-E2 are predominantly nuclear with specific enrichment of hnRNP-E1 in nuclear speckles. Among the hnRNPE family, hnRNP-E1 and hnRNP-E2 share the highest amino acid sequence similarity. PCBP1 is ubiquitously expressed and is located on human chromosome 2p13.3.

Especificidad

Anti-hnRNP-E1 (C-terminal) (also known as PCBP1/2 and αCP1) specifically recognizes human, mouse, and rat hnRNP-E1.

Aplicación

Anti-hnRNP-E1 (C-terminal) antibody produced in rabbit may be used in immunoblotting[1] and immunoprecipitation.

Acciones bioquímicas o fisiológicas

Heterogeneous nuclear ribonucleoproteins (hnRNPs) are important in biological activities such as transcription, pre-mRNA processing, mRNA translation and turnover. hnRNPE proteins are also implicated in mRNA stabilization, translation enhancement and translation silencing. Heterogeneous nuclear ribonucleoprotein E1 (hnRNP E1)/poly(rC)-binding protein 1 (PCBP1) acts as a tumor-suppressive splicing factor. Upregulation of this gene can repress the tumorigenicity of ovarian cancer, prostate cancer, gastric cancer, colon cancer and thyroid carcinoma cells.

Forma física

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Almacenamiento y estabilidad

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)