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P9424

Proteína A – Agarosa

aqueous ethanol suspension

Sinónimos:

Protein A–Agarose

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A ustedes/SKUDisponibilidadPrecio
1 mL
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352,00 €
5 mL
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1190,00 €

Acerca de este artículo

UNSPSC Code:
41106500
NACRES:
NA.56
MDL number:
Form:
aqueous ethanol suspension
Biological source:
Staphylococcus aureus

352,00 €


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biological source

Staphylococcus aureus

Quality Segment

form

aqueous ethanol suspension

analyte chemical class(es)

proteins (Immunoglobulins of various mammalian species)

extent of labeling

~6 mg per mL

technique(s)

affinity chromatography: suitable

matrix

Sepharose 4B Fast Flow

matrix activation

cyanogen bromide

matrix attachment

amino

matrix spacer

1 atom

capacity

≥30 mg/mL binding capacity (human IgG)

storage temp.

2-8°C

General description

Protein A-Sepharose has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.[1]

Application

Protein A-Sepharose is used for affinity chromatography, antibody purification and characterization, immunoaffinity matrices, protein chromatography, protein A, G and L resins, and recombinant protein expression and analysis. Protein A-Sepharose has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.
Protein A-Sepharose Fast Flow from Staphylococcus aureus has been used:
  • in co-immunoprecipitation assay[2]
  • in immunodepletion[3]
  • to purify IgG from human serum and plasma[3]

Physical form

Suspension in 20% ethanol

Legal Information

Sepharose is a trademark of Cytiva

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Este artículo
H9395P3391P3296
technique(s)

affinity chromatography: suitable

technique(s)

-

technique(s)

affinity chromatography: suitable

technique(s)

affinity chromatography: suitable

biological source

Staphylococcus aureus

biological source

Staphylococcus aureus

biological source

-

biological source

-

form

aqueous ethanol suspension

form

lyophilized powder

form

lyophilized powder

form

aqueous ethanol suspension

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

100

Quality Level

300

Quality Level

200

Quality Level

200

matrix

Sepharose 4B Fast Flow

matrix

-

matrix

Sepharose CL-4B

matrix

Sepharose 4B Fast Flow


pictograms

Flame

signalword

Warning

hcodes

Hazard Classifications

Flam. Liq. 3

wgk

WGK 3

Clase de almacenamiento

3 - Flammable liquids

flash_point_f

104.0 °F

flash_point_c

40 °C



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Preguntas

  1. For ChIP-seq experiments, what is the correct procedure to prepare a 50% Protein A–Sepharose bead slurry? Specifically, can the slurry be prepared by pelleting 20 µL of the stock beads at 1,000 g for 30 s, discarding the storage buffer, and resuspending

    1 respuesta
    1. Please consult the User Guide for all procedures recommended while using this product. If the information is not in the User Guide, we have not tested other conditions out. Link: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/306/054/p9424pis-ms.pdf

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