Using P5282 with cells fixed in paraformaldehyde instead of methanol-free formaldehyde was attempted, but the results were not satisfactory with regular formaldehyde. A specific protocol for the reconstitution of Phalloidin P5282-.1MG in the case of tissues fixed in paraformaldehyde and included in O.C.T medium is not available. However, it is suggested to find relevant information in the literature, such as in this article, https://www.oncotarget.com/article/25259/ DOI:[10.18632/oncotarget.25259].
P5282
Phalloidin Peptide
≥90% (HPLC), solid, FITC labeled
Sinónimos:
Phalloidin-FITC
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0.1 MG
510,00 €
510,00 €
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0.1 MG
510,00 €
About This Item
Fórmula empírica (notación de Hill):
C56H60N10O15S2
Peso molecular:
1177.26
Número MDL:
Código UNSPSC:
12352202
NACRES:
NA.32
510,00 €
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Nombre del producto
Phalloidin, Fluorescein Isothiocyanate Labeled, sequence Amanita phalloides(synthetic: peptide sequence)
origen biológico
sequence from Amanita phalloides (synthetic: peptide sequence)
Nivel de calidad
Formulario
solid
fluorescencia
λex 495 nm; λem 520 nm(lit.)
temp. de almacenamiento
−20°C
Descripción general
Phalloidin is a phallotoxin produced by death cap mushroom Amanita phalloides. It is a cyclic peptide, which interacts with actin and this was first identified in phalloidin-poisoned rats. It is a heptapeptide, cyclic in nature, with a crosslink between tryptophan at position 6 and cysteine at position 3.[1] The side chain of amino acid 7 (γ-δ-dihydroxyleucine) in phalloidin, is accessible to modifications, through which fluorescently labelled phalloidin compounds can be produced.[2]
Aplicación
Phalloidin, Fluorescein Isothiocyanate Labeled has been used:
Acciones bioquímicas o fisiológicas
Phalloidin interacts with polymeric actin, and not oligomeric or monomeric forms. This interaction leads to highly stabilized actin filaments, which resist depolymerization and disassembly. In rats, this toxin causes death due to liver hemorrhage, and cells show abnormal actin clustering.[1] The affinity of phalloidin to actin is not significantly altered after derivatizing fluorescently labelled phalloidin compounds. These compounds can be used to study actin structure and organization within eukaryotic cells.[2]
Toxin that binds polymeric F actin, stabilizing it and interfering with the function of actin-rich structures.
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Acute Tox. 2 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral
Código de clase de almacenamiento
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Certificados de análisis (COA)
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Artículos
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Contenido relacionado
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Is it possible to use P5282 with cells fixed in paraformaldehyde instead of methanol-free formaldehyde?
Is it possible to use P5282 with cells fixed in paraformaldehyde instead of methanol-free formaldehyde?
1 respuesta-
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How do I prepare stock solutions of Product P5282, Phalloidin, Fluorescein Isothiocyanate Labeled?
1 respuesta-
Stock solutions of phalloidin conjugates have been made in methanol or DMSO at 0.1 to 5 mg/mL. Make final dilutions in aqueous physiological buffers for a staining range from 0.1 μM to 100 μM with corresponding incubation times of 15 minutes to 72 hours.
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What is the Department of Transportation shipping information for this product?
1 respuesta-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How do I store Product P5282, Phalloidin, Fluorescein Isothiocyanate Labeled?
1 respuesta-
The product should be stored at a powder in the freezer at -20°C.
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How do I dissolve Product P5282, Phalloidin, Fluorescein Isothiocyanate Labeled?
1 respuesta-
This product is soluble in methanol, ethanol, butanol and pyridine. It is soluble in cold water at a concentration of 5 mg/mL; it is much more soluble in hot water. See: The chemicals encyclopedia published by the Royal Society of Chemistry, 12th ed., entry# 7336 (1996). Solutions should be prepared fresh, and protected from light whenever possible.
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How do I stain cells with Product P5282, Phalloidin, Fluorescein Isothiocyanate Labeled?
1 respuesta-
A typical application for staining cells can be found in the product information sheet (under Documents, above).
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What are the excitation and emission wavelengths used for fluorescent detection of Product P5282, Phalloidin, Fluorescein Isothiocyanate Labeled?
1 respuesta-
Excitation wavelengths of 540-545 nm and emission wavelengths of 570-573 nm can be used. See: Faulstich, H., J. Muscle Res. Cell Motility, 9, 370 (1988), and Waggoner, A. et al., Methods in Cell Biology, 30, 449 (1989).
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