N9284
Nitroreductase from Escherichia coli
≥90% (SDS-PAGE), recombinant, expressed in E. coli
Sinónimos:
NTRA
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recombinante
expressed in E. coli
Nivel de calidad
Análisis
≥90% (SDS-PAGE)
formulario
lyophilized powder
actividad específica
≥100 units/mL
mol peso
monomer 24000
características de los productos alternativos más sostenibles
Waste Prevention
Safer Solvents and Auxiliaries
Design for Energy Efficiency
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sustainability
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Nº de acceso UniProt
categoría alternativa más sostenible
Condiciones de envío
wet ice
temp. de almacenamiento
−20°C
Información sobre el gen
Escherichia coli K12 ... nfsB(945483)
Categorías relacionadas
Descripción general
Nitroreductase is a flavoprotein and is encoded by the NfsB gene. It comprises a dimer, with 217 amino acids and active site in each subunit. The structure has FMN and the substrate bound to the enzyme.
Aplicación
Nitroreductase from Escherichia coli has been used in the conjugation generation with pig liver esterase (PLE). It has also been used in chemiluminescence response studies with probes HyCL-3 and HyCL-4-AM in rat liver microsomes.
Acciones bioquímicas o fisiológicas
Nitroreductase (NTR) catalyzes the reduction of nitroaromatic substrates and quinones. The mutant F124K of NTR is useful in cancer therapy and improves sensitization of drug CB1954.
Nitroreductase increases the sensitivity of organisms to nitro-containing drugs such as metronidazole by converting the nitro group to a cytotoxic nitro radical.
Nitroreductases can play a crucial role in redox systems via NADPH or NADH as a reductant.
Shows ability to reduce quinines. Enzyme for activating prodrugs in antibody directed enzyme prodrug therapy.
Propiedades físicas
Lyophilized powder containing PBS. Does not contain BSA as excipient
Definición de unidad
One unit will reduce one μmole of Cytochrome C per minute in the presence of Menadione and NADH at pH 7.4 at 37 °C.
Nota de preparación
Produced using animal component-free materials.
Código de clase de almacenamiento
13 - Non Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
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