The product is presented as a formulated preparation, not a highly purified nisin. The specification ≥900 IU/mg reflects potency. Nisin content is approximately 2.5% (w/w) in this product, with the remainder consisting of stabilizing agents and carriers.
N5764
Nisin from Lactococcus lactis
potency: ≥900 IU/mg
Sinónimos:
Nisin A
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| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
1 g | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 64,20 € |
5 g | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 205,00 € |
25 g | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 758,00 € |
Acerca de este artículo
Fórmula empírica (notación de Hill):
C143H230N42O37S7
Número CAS:
Peso molecular:
3354.07
NACRES:
NA.85
PubChem Substance ID:
UNSPSC Code:
51102829
MDL number:
64,20 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
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powder
Quality Segment
potency
≥900 IU/mg
antibiotic activity spectrum
Gram-negative bacteria, Gram-positive bacteria
mode of action
cell membrane | interferes
storage temp.
2-8°C
SMILES string
[H]N[C@@H]([C@@H](C)CC)C(=O)N\C(=C\C)C(=O)N[C@H]1CSC[C@H](NC(=O)[C@H](CC(C)C)NC(=O)C(=C)NC(=O)[C@@H](NC1=O)[C@@H](C)CC)C(=O)N[C@H]2[C@H](C)SC[C@H](NC(=O)CNC(=O)[C@@H]3CCCN3C2=O)C(=O)N[C@H](CCCCN)C(=O)N[C@@H]4[C@@H](C)SC[C@H](NC(=O)CNC(=O)[C@H](CCSC)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)CNC4=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCCN)C(=O)N[C@H]5[C@H](C)SC[C@@H]6NC(=O)[C@@H](NC(=O)[C@H](C)NC5=O)[C@H](C)SC[C@H](NC(=O)[C@H](Cc7cnc[nH]7)NC6=O)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](Cc8cnc[nH]8)C(=O)N[C@@H](C(C)C)C(=O)NC(=C)C(=O)N[C@@H](CCCCN)C(O)=O
InChI
1S/C143H230N42O37S7/c1-24-69(11)105(148)135(213)162-82(27-4)118(196)174-94-58-225-59-95(175-123(201)89(48-67(7)8)169-115(193)74(16)158-138(216)107(70(12)25-2)180-132(94)210)133(211)184-112-79(21)229-61-96(160-104(190)56-152-134(212)100-38-34-44-185(100)142(112)220)128(206)164-84(36-29-32-42-145)120(198)182-109-76(18)226-60-97(161-103(189)55-151-117(195)85(39-45-223-22)165-122(200)88(47-66(5)6)168-113(191)72(14)156-102(188)54-153-136(109)214)129(207)171-92(51-101(147)187)125(203)166-86(40-46-224-23)119(197)163-83(35-28-31-41-144)121(199)183-110-77(19)228-63-99-130(208)170-90(49-80-52-149-64-154-80)124(202)176-98(62-227-78(20)111(141(219)177-99)181-116(194)75(17)159-140(110)218)131(209)173-93(57-186)127(205)179-108(71(13)26-3)139(217)172-91(50-81-53-150-65-155-81)126(204)178-106(68(9)10)137(215)157-73(15)114(192)167-87(143(221)222)37-30-33-43-146/h27,52-53,64-72,75-79,83-100,105-112,186H,15-16,24-26,28-51,54-63,144-146,148H2,1-14,17-23H3,(H2,147,187)(H,149,154)(H,150,155)(H,151,195)(H,152,212)(H,153,214)(H,156,188)(H,157,215)(H,158,216)(H,159,218)(H,160,190)(H,161,189)(H,162,213)(H,163,197)(H,164,206)(H,165,200)(H,166,203)(H,167,192)(H,168,191)(H,169,193)(H,170,208)(H,171,207)(H,172,217)(H,173,209)(H,174,196)(H,175,201)(H,176,202)(H,177,219)(H,178,204)(H,179,205)(H,180,210)(H,181,194)(H,182,198)(H,183,199)(H,184,211)(H,221,222)/b82-27+/t69-,70-,71-,72-,75-,76?,77?,78?,79?,83-,84+,85-,86-,87-,88-,89-,90-,91-,92-,93-,94-,95-,96-,97-,98-,99-,100-,105-,106-,107-,108-,109+,110-,111-,112-/m0/s1
InChI key
NVNLLIYOARQCIX-ILAIHEEGSA-N
General description
Chemical structure: peptide
Application
Nisin is a polycyclic lantibiotic produced by Lactococcus lactis. It is commonly used as a food preservative. It is also used as a selective agent in cell culture for the isolation of gram-negative bacteria, yeast, and molds. Nisin has been used in bioassays to study posttranslational modification.
Used to form targeted pores in cell membranes that are mediated by lipid II. Used to study the assembly and stability of nisin-lipid II pore complexes.
Biochem/physiol Actions
Nisin binds to cell wall precursor lipid components of bacteria and disrupts cell wall production. Nisin alters the cell membrane which results in the leakage of cytoplasmic components and destruction of the proton motive force.
Nisin is an antibiotic with bactericidal action. It binds to the lipid A portion of bacterial lipopolysaccharides and induces pore formation in the membranes of cortex cells from excised sorghum roots.
Mode of Action: Binds to and interferes with the permeability of the cytoplasmic membrane.
Antimicrobial spectrum: Gram-negative and Gram-positive bacteria (requires chelator such as EDTA).
Mode of Action: Binds to and interferes with the permeability of the cytoplasmic membrane.
Antimicrobial spectrum: Gram-negative and Gram-positive bacteria (requires chelator such as EDTA).
Packaging
Package size based on weight of total solids
Other Notes
Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.
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Este artículo | |||
|---|---|---|---|
| description potency: ≥900 IU/mg | description Ready Made Solution, (20,000-40,000 IU/mL in 0.02N HCl) | description ~11,500 U/mg | description aminoglycoside antibiotic |
| antibiotic activity spectrum Gram-negative bacteria, Gram-positive bacteria | antibiotic activity spectrum Gram-positive bacteria | antibiotic activity spectrum Gram-negative bacteria | antibiotic activity spectrum Gram-negative bacteria, Gram-positive bacteria, mycoplasma, mycobacteria |
| mode of action cell membrane | interferes | mode of action cell membrane | interferes | mode of action cell membrane | interferes | mode of action protein synthesis | interferes |
| Quality Level 200 | Quality Level - | Quality Level 200 | Quality Level 200 |
| form powder | form solution, ready-to-use solution | form powder | form powder |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. - |
| potency ≥900 IU/mg | potency - | potency - | potency ~650 μg per mg |
Clase de almacenamiento
11 - Combustible Solids
¿Qué está pasando?
WGK 3
Punto de inflamación (°F)
Not applicable
Punto de inflamación (°C)
Not applicable
EPI (equipos de protección individual)
Eyeshields, Gloves, type N95 (US)
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I hope this message finds you well. I am currently considering the purchase of your Nisin (catalog number: N5764) for my research. The product page indicates a potency of ≥900 IU/mg, but I was unable to find specific information regarding the purity (%)
1 respuesta-
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Does the product come sterile? If so, how has it been sterilised?
1 respuesta-
This product is not sterile. However, it is tested to confirm the absence of Staphylococcus aureus, Salmonella, and Escherichia coli.
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf¿Le ha resultado útil?
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Does this product ,nisin powder concentration: 2.5% (w/w) or pure nisin ?
1 respuesta-
Please see the composition of this product below:
Nisin - 2.5%
Sodium Chloride - 90%
Protein - 4%
Carbohydrate - 1.5%
Moisture - 2%¿Le ha resultado útil?
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What is the production strain of this product? Lactococcus lactis subsp. lactis ATCC 11454?
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The subclass of Lactococcus lactis is not provided.
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Is It Food grade E234
1 respuesta-
This product is intended for research use only and is not suitable for human or veterinary use. The product is not food grade material.
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Bonjour, Avec quoi peut on re-suspendre ce produit? Peut on le stocker après re-suspension? Merci par avance
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The solubility has not been tested. However, several literature sources indicate that this material is soluble in dilute acids. Solubility was quite high at low pH (57 mg/ml at pH 2) and much lower at high pH (0.25 mg/ml at pH 8 to 12). Dissolving the compound in water is not recommended. In addition, the product may also be soluble in DMSO with gentle warming and ultrasonication, up to at least 5 mg/mL. This information has not been validated. Please see the link below to review a publication that may be of interest:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC184764/¿Le ha resultado útil?
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Does Product No N5764, Nisin contain denatured milk solids?
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This product does not contain any milk solids
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