ExoPolyzyme

Name: ExoPolyzyme
Brand: SIGMA

For enzymatic lysis of extracellular biofilm matrix

Sinónimos:

Biofilm degrading enzyme mix, Extracellular matrix degrading enzymatic mixture

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Acerca de este artículo

NACRES:

NA.32

UNSPSC Code:

42312305

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Propiedades

Quality Segment

200

form

solid

packaging

pkg of 1 vial

input

microbiome, soil and stool sample(s)

shipped in

dry ice

storage temp.

-10 to -25°C

Descripción

General description

Biofilms are complex structures, which adhere to surfaces and comprise of one or more types of bacteria and fungi. They form an extracellular matrix (ECM) which is generally composed of secreted polymers such as exopolysaccharides (EPS), extracellular DNA (eDNA), peptides and lipids. ExoPolyzyme is a mixture of seven enzymes that break down different components residing in ECM, such as EPS’s and lipids. Each of these enzymes has been previously used to inhibit or remove biofilm. The original development was in collaboration with Scott Tighe and the Extreme Microbiome Project.

Application

ExoPolyzme can be used in biofilm research and as a gentler method for removing the EPS and lipids from the ECM in metagenomic protocols, providing better access to the bacterial cells within the biofilm and eDNA.

Features and Benefits

Mixture of seven enzymes that break down different components residing in ECM, such as EPS’s and lipids
Gently removes the EPS and lipids from the ECM in metagenomic protocols
Allows better access to the bacterial cells within the biofilm and eDNA

Other Notes

The components in ExoPolyzyme (MBD0064) are:

α-Amylase
Cellulase
β-Glucosidase
Lyticase
Alginate lyase
Lipase
Hemicellulase

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Este artículo	MAC4L	SAE0200	MAC4LDF
Sigma-Aldrich MBD0064 ExoPolyzyme	Sigma-Aldrich MAC4L MetaPolyzyme	Sigma-Aldrich SAE0200 MycoPolyzyme	Sigma-Aldrich MAC4LDF MetaPolyzyme, DNA free
description For enzymatic lysis of extracellular biofilm matrix	description lyophilized powder	description for use with yeast and fungal lysis, free of DNA contaminants, suitable for Microbiome research	description Suitable for Microbiome research, lyophilized powder
form solid	form lyophilized powder	form lyophilized powder	form lyophilized powder
storage temp. -10 to -25°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
shipped in dry ice	shipped in wet ice	shipped in wet ice	shipped in wet ice
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
input microbiome, soil and stool sample(s)	input microbiome, soil and stool sample(s)	input -	input microbiome, soil and stool sample(s)
packaging pkg of 1 vial	packaging -	packaging -	packaging -